PDF(Pubmed)
Abstract：
The Oriental hornet (Vespa orientalis) is one of the major predators of honey bees. It has been demonstrated that adults of V. orientalis can harbor honey bee viruses, however the transmission route of infection is still not clear. The aim of this study was to study the possible presence of honey bee viruses in V. orientalis larvae and honey bees collected from the same apiary. Therefore, 29 samples of V. orientalis larvae and 2 pools of honey bee (Apis mellifera). samples were analyzed by multiplex PCR to detect the presence of six honeybee viruses: Acute Bee Paralysis Virus (ABPV), Black Queen Cell Virus (BQCV), Chronic Bee Paralysis Virus (CBPV), Deformed Wing Virus (DWV), Kashmir Bee Virus (KBV) and Sac Brood Virus (SBV). Biomolecular analysis of V. orientalis larvae revealed that DWV was present in 24/29 samples, SBV in 10/29, BQCV in 7/29 samples and ABPV in 5/29 samples, while no sample was found positive for CBPV or KBV. From biomolecular analysis of honey bee samples DWV was the most detected virus, followed by SBV, BQCV, ABPV. No honey bee sample was found positive for CBPV or KBV. Considering the overlapping of positivities between V.orientalis larvae and honey bee samples, and that V.orientalis larvae are fed insect proteins, preferably honey bees, we can suggest the acquisition of viral particles through the ingestion of infected bees. However, future studies are needed to confirm this hypothesis and rule out any other source of infection.
摘要：
东方大黄蜂（Vespaorientalis）是蜜蜂的主要捕食者之一。已经证明，东方弧菌的成虫可以携带蜜蜂病毒，然而，感染的传播途径仍然不清楚。这项研究的目的是研究从同一养蜂场采集的东方弧菌幼虫和蜜蜂中可能存在的蜜蜂病毒。因此,29个东方弧菌幼虫样品和2个蜜蜂池（Apismellifera）。通过多重PCR分析样品以检测六种蜜蜂病毒的存在：急性蜜蜂麻痹病毒（ABPV），黑皇后细胞病毒(BQCV),慢性蜜蜂麻痹病毒（CBPV），机翼变形病毒(DWV),克什米尔蜜蜂病毒（KBV）和SacBrood病毒（SBV）。东方弧菌幼虫的生物分子分析表明，DWV存在于24/29个样品中，SBV为10/29，BQCV为7/29，ABPV为5/29，而没有发现CBPV或KBV阳性的样本。从蜜蜂样品的生物分子分析中，DWV是检测到最多的病毒，其次是SBV，BQCV,ABPV。没有发现蜜蜂样品对CBPV或KBV呈阳性。考虑到东方黄蜂幼虫和蜜蜂样本之间的阳性重叠，东方神虫幼虫被喂食昆虫蛋白，最好是蜜蜂，我们可以建议通过摄入受感染的蜜蜂来获取病毒颗粒。然而,未来的研究需要证实这一假设,并排除任何其他感染源.
