Abstract：
RuvBL, is a member of SF6 superfamily of helicases and is conserved among the various model systems. Recently, rice (Oryza sativa L.) homolog of RuvBL has been biochemically characterized for its ATPase and DNA helicase activities; however its involvement in stress has not been studied so far. Present investigation reports the detailed functional characterization of OsRuvBL under abiotic stresses through genetic engineering. An efficient Agrobacterium-mediated in planta transformation protocol was developed in indica rice to generate the transgenic lines and study was focused on optimization of factors to achieve maximum transformation efficiency. Overexpressing OsRuvBL1a transgenic lines showed enhanced tolerance under in vivo salinity stress as compared to WT plants. The physiological and biochemical analysis of the OsRuvBL1a transgenic lines showed better performance under salinity and drought stresses. Several stress responsive interacting partners of OsRuvBL1a were identified using Y2H system revealed to its role in stress tolerance. Functional mechanism for boosting stress tolerance by OsRuvBL1a has been proposed in this study. This integration of OsRuvBL1a gene in rice genome using in planta transformation method helped to achieve the abiotic stress resilient smart crop. This study is the first direct evidence to show the novel function of RuvBL in boosting abiotic stress tolerance in plants.
摘要：
RuvBL,是解旋酶的SF6超家族的成员，在各种模型系统中保守。最近,RuvBL的水稻（OryzasativaL.）同系物已在生物化学上表征了其ATPase和DNA解旋酶活性；但是到目前为止，尚未研究其与胁迫的关系。本研究报告了通过基因工程在非生物胁迫下OsRuvBL的详细功能表征。在in稻中开发了一种有效的农杆菌介导的植物转化方案，以产生转基因品系，并且研究的重点是优化因子以实现最大的转化效率。与WT植物相比，过表达OsRuvBL1a转基因品系在体内盐度胁迫下显示出增强的耐受性。OsRuvBL1a转基因品系的生理生化分析表明，在盐度和干旱胁迫下表现更好。使用Y2H系统鉴定了OsRuvBL1a的几个应激反应相互作用伙伴，揭示了其在胁迫耐受性中的作用。本研究提出了OsRuvBL1a增强应激耐受性的功能机制。利用植物转化方法将OsRuvBL1a基因整合到水稻基因组中，有助于实现作物的抗逆性。这项研究是显示RuvBL在提高植物非生物胁迫耐受性方面的新功能的第一个直接证据。
