Abstract：
Detection of viable viruses in the air is critical in order to determine the level of risk associated with the airborne diffusion of viruses. Different methods have been developed for the isolation, purification, and detection of viable airborne viruses, but they require an extensive processing time and often present limitations including low physical efficiency (i.e., the amount of collected viruses), low biological efficiency (i.e., the number of viable viruses), or a combination of all. To mitigate such limitations, we have employed an efficient technique based on the magnetic levitation (Maglev) technique with a paramagnetic solution and successfully identified distinct variations in levitation and density characteristics among bacteria (Escherichia coli), phages (MS2), and human viruses (SARS-CoV-2 and influenza H1N1). Notably, the Maglev approach enabled a significant enrichment of viable airborne viruses in air samples. Furthermore, the enriched viruses obtained through Maglev exhibited high purity, rendering them suitable for direct utilization in subsequent analyses such as reverse transcription-polymerase chain reaction (RT-PCR) or colorimetric assays. The system is portable, easy to use, and cost-efficient and can potentially provide proactive surveillance data for monitoring future outbreaks of airborne infectious diseases and allow for the induction of various preventative and mitigative measures.
摘要：
检测空气中的活病毒对于确定与病毒的空气传播相关的风险水平至关重要。已经开发了不同的隔离方法，净化,并检测空气中的病毒，但它们需要大量的处理时间，并且经常存在限制，包括低物理效率(即，收集的病毒数量)，低生物效率(即，活病毒的数量)，或所有的组合。为了减轻这种限制，我们采用了一种有效的技术，基于磁悬浮（磁悬浮）技术与顺磁性溶液，并成功地确定了细菌（大肠杆菌）之间的悬浮和密度特征的不同变化，噬菌体（MS2），和人类病毒（SARS-CoV-2和H1N1流感）。值得注意的是,磁悬浮方法使空气样品中的活空气病毒大量富集。此外，通过磁悬浮获得的富集病毒表现出高纯度，使它们适合在随后的分析中直接使用，例如逆转录聚合酶链反应（RT-PCR）或比色测定。该系统是便携式的，易于使用,和成本效益高，并有可能提供主动监测数据，以监测未来的空气传播传染病暴发，并允许采取各种预防和缓解措施。
