Abstract：
This study assessed the antimicrobial effect of sodium hypochlorite (NaOCl) mixtures combined with Keratobacter (KB) using an engineered biofilm root canal model. Clinical and reagent grade NaOCl were mixed with KB (9:1-vol/vol) to assess pH values over 1 min to select the ideal solution with a pH just below the pKa of hypochlorous acid. The samples were randomly divided into five groups: 1% and 4% NaOCl reagents, a mixture of NaOCl:KB using 1% and 4% NaOCl reagents and distilled water. Outcome measures were colony-forming units (CFUs/mL) and positive/negative cultures. No significant differences were observed in the pairwise comparisons between 1%, 4% NaOCl and 4% NaOCl+KB for the outcome CFUs/mL. Only 4% NaOCl presented with negative cultures in all samples, whereas 1% NaOCl and 4% NaOCl+KB had similar results (54% vs. 40%). The addition of KB has a limited effect on the antimicrobial efficacy of 4% NaOCl in this laboratory model.
摘要：
这项研究使用工程生物膜根管模型评估了次氯酸钠（NaOCl）混合物与角化杆菌（KB）的抗菌作用。将临床和试剂级NaOCl与KB（9：1-vol/vol）混合以评估1分钟内的pH值，以选择pH刚好低于次氯酸pKa的理想溶液。将样品随机分为5组:1%和4%NaOCl试剂,使用1%和4%NaOCl试剂和蒸馏水的NaOCl:KB的混合物。结果测量为菌落形成单位(CFU/mL)和阳性/阴性培养物。在1%之间的成对比较中没有观察到显著差异，4%NaOCl和4%NaOCl+kb为成果CFUs/mL。所有样品中只有4%NaOCl呈阴性培养物，而1%NaOCl和4%NaOCl+KB的结果相似(54%与40%）。在该实验室模型中,KB的添加对4%NaOCl的抗微生物功效具有有限的影响。
