Abstract：
mRNA vaccines have been developed as a promising cancer management. It is noted that specification of the antigen sequence of the target antigen is necessary for the design and manufacture of an mRNA vaccine.
The steps involved in preparing the mRNA-based cancer vaccines are isolation of the mRNA cancer from the target protein using the nucleic acid RNA-based vaccine, sequence construction to prepare the DNA template, in vitro transcription for protein translation from DNA into mRNA strand, 5\' cap addition and poly(A) tailing to stabilize and protect the mRNA from degradation and purification process to remove contaminants produced during preparation.
Lipid nanoparticles, lipid/protamine/mRNA nanoparticles, and cell-penetrating peptides have been used to formulate mRNA vaccine and to ensure vaccine stability and delivery to the target site. Delivery of the vaccine to the target site will trigger adaptive and innate immune responses. Two predominant factors of the development of mRNA-based cancer vaccines are intrinsic influence and external influence. In addition, research relating to the dosage, route of administration, and cancer antigen types have been observed to positively impact the development of mRNA vaccine.
The steps involved in preparing the mRNA-based cancer vaccines are isolation of the mRNA cancer from the target protein using the nucleic acid RNA-based vaccine, sequence construction to prepare the DNA template, in vitro transcription for protein translation from DNA into mRNA strand, 5\' cap addition and poly(A) tailing to stabilize and protect the mRNA from degradation and purification process to remove contaminants produced during preparation.
Lipid nanoparticles, lipid/protamine/mRNA nanoparticles, and cell-penetrating peptides have been used to formulate mRNA vaccine and to ensure vaccine stability and delivery to the target site. Delivery of the vaccine to the target site will trigger adaptive and innate immune responses. Two predominant factors of the development of mRNA-based cancer vaccines are intrinsic influence and external influence. In addition, research relating to the dosage, route of administration, and cancer antigen types have been observed to positively impact the development of mRNA vaccine.
摘要：
■mRNA疫苗已被开发为有希望的癌症管理。应注意,靶抗原的抗原序列的说明对于mRNA疫苗的设计和制造是必要的。
制备基于mRNA的癌症疫苗所涉及的步骤是使用基于核酸RNA的疫苗从靶蛋白中分离mRNA癌症，序列构建以制备DNA模板，蛋白质从DNA翻译成mRNA链的体外转录，5'帽添加和poly（A）加尾，以稳定和保护mRNA免于降解和纯化过程，以去除制备过程中产生的污染物。
■脂质纳米颗粒，脂质/鱼精蛋白/mRNA纳米粒，和细胞穿透肽已被用于配制mRNA疫苗并确保疫苗稳定性和递送至靶位点。将疫苗递送到靶位点将触发适应性和先天免疫应答。基于mRNA的癌症疫苗开发的两个主要因素是内在影响和外部影响。此外,有关剂量的研究，给药途径,已经观察到癌症抗原类型对mRNA疫苗的开发产生了积极影响。
制备基于mRNA的癌症疫苗所涉及的步骤是使用基于核酸RNA的疫苗从靶蛋白中分离mRNA癌症，序列构建以制备DNA模板，蛋白质从DNA翻译成mRNA链的体外转录，5'帽添加和poly（A）加尾，以稳定和保护mRNA免于降解和纯化过程，以去除制备过程中产生的污染物。
■脂质纳米颗粒，脂质/鱼精蛋白/mRNA纳米粒，和细胞穿透肽已被用于配制mRNA疫苗并确保疫苗稳定性和递送至靶位点。将疫苗递送到靶位点将触发适应性和先天免疫应答。基于mRNA的癌症疫苗开发的两个主要因素是内在影响和外部影响。此外,有关剂量的研究，给药途径,已经观察到癌症抗原类型对mRNA疫苗的开发产生了积极影响。
