PDF(Pubmed)
Abstract：
UNASSIGNED: In this study, we conducted an integrated study of the diagnostic value of MiR-223 in ectopic pregnancy (EP).
UNASSIGNED: We used GSE44731 downloaded from GEO and GEO2R to identify differentially expressed miRNA. The hub genes corresponding to the differential miRNA were then identified by using the Xiantao academic tool, GO (Gene Ontology), and KEGG (Kyoto Encyclopedia of Genes and Genomes). Afterward, we used the miEAA database to perform gene set enrichment analysis (GSEA) of differential miRNA, and used Xiantao academic tools again to conduct the ceRNA network based on the target genes. Protein-protein interaction (PPI) network construction and lncRNA of hub miRNA target genes were then predicted by the starbase database. For validation, the villus tissue from intrauterine pregnancy and tubal pregnancy was collected and assayed by qPCR.
UNASSIGNED: In total 19 differentially expressed miRNAs were screened out, of which MiR-223 had a relatively clear diagnostic significance. Hub genes were enriched and analyzed by GO, KEGG, and GSEA, and the results showed that regulation of NF-κB and other signaling pathways are primarily enriched in ectopic pregnancy. We also obtained 215 key genes from PPI analysis. Our ceRNA analysis indicated that LRRC75A-AS1 and PITPNA-AS1 were associated with MiR-223, and the expression of MiR-223 in qPCR was significantly high in tubal pregnancy group.
UNASSIGNED: We found that MiR-223 can be used in the diagnosis of EP. Our findings provide valuable information and direction for future research into novel targets for EP diagnosis.
UNASSIGNED: We used GSE44731 downloaded from GEO and GEO2R to identify differentially expressed miRNA. The hub genes corresponding to the differential miRNA were then identified by using the Xiantao academic tool, GO (Gene Ontology), and KEGG (Kyoto Encyclopedia of Genes and Genomes). Afterward, we used the miEAA database to perform gene set enrichment analysis (GSEA) of differential miRNA, and used Xiantao academic tools again to conduct the ceRNA network based on the target genes. Protein-protein interaction (PPI) network construction and lncRNA of hub miRNA target genes were then predicted by the starbase database. For validation, the villus tissue from intrauterine pregnancy and tubal pregnancy was collected and assayed by qPCR.
UNASSIGNED: In total 19 differentially expressed miRNAs were screened out, of which MiR-223 had a relatively clear diagnostic significance. Hub genes were enriched and analyzed by GO, KEGG, and GSEA, and the results showed that regulation of NF-κB and other signaling pathways are primarily enriched in ectopic pregnancy. We also obtained 215 key genes from PPI analysis. Our ceRNA analysis indicated that LRRC75A-AS1 and PITPNA-AS1 were associated with MiR-223, and the expression of MiR-223 in qPCR was significantly high in tubal pregnancy group.
UNASSIGNED: We found that MiR-223 can be used in the diagnosis of EP. Our findings provide valuable information and direction for future research into novel targets for EP diagnosis.
摘要：
■在这项研究中，我们对MiR-223在异位妊娠(EP)中的诊断价值进行了综合研究.
■我们使用从GEO和GEO2R下载的GSE44731来鉴定差异表达的miRNA。然后利用仙桃学术工具鉴定与差异miRNA对应的hub基因,GO（基因本体论），和KEGG（京都基因和基因组百科全书）。之后,我们使用miEAA数据库对差异miRNA进行基因集富集分析(GSEA),并再次使用仙桃学术工具进行基于目标基因的ceRNA网络。然后通过starbase数据库预测hubmiRNA靶基因的蛋白质-蛋白质相互作用（PPI）网络构建和lncRNA。对于验证，收集宫内妊娠和输卵管妊娠的绒毛组织，并通过qPCR进行检测。
■共筛选出19个差异表达的miRNA，其中MiR-223具有相对明确的诊断意义。Hub基因通过GO进行富集和分析，KEGG,还有GSEA,结果表明，NF-κB和其他信号通路的调节主要集中在异位妊娠中。我们还从PPI分析中获得了215个关键基因。我们的ceRNA分析表明LRRC75A-AS1和PITPNA-AS1与MiR-223相关，并且在qPCR中MiR-223的表达在输卵管妊娠组中明显高。
■我们发现MiR-223可用于EP的诊断。我们的发现为将来研究EP诊断的新目标提供了有价值的信息和方向。
■我们使用从GEO和GEO2R下载的GSE44731来鉴定差异表达的miRNA。然后利用仙桃学术工具鉴定与差异miRNA对应的hub基因,GO（基因本体论），和KEGG（京都基因和基因组百科全书）。之后,我们使用miEAA数据库对差异miRNA进行基因集富集分析(GSEA),并再次使用仙桃学术工具进行基于目标基因的ceRNA网络。然后通过starbase数据库预测hubmiRNA靶基因的蛋白质-蛋白质相互作用（PPI）网络构建和lncRNA。对于验证，收集宫内妊娠和输卵管妊娠的绒毛组织，并通过qPCR进行检测。
■共筛选出19个差异表达的miRNA，其中MiR-223具有相对明确的诊断意义。Hub基因通过GO进行富集和分析，KEGG,还有GSEA,结果表明，NF-κB和其他信号通路的调节主要集中在异位妊娠中。我们还从PPI分析中获得了215个关键基因。我们的ceRNA分析表明LRRC75A-AS1和PITPNA-AS1与MiR-223相关，并且在qPCR中MiR-223的表达在输卵管妊娠组中明显高。
■我们发现MiR-223可用于EP的诊断。我们的发现为将来研究EP诊断的新目标提供了有价值的信息和方向。
