PDF(Pubmed)
Abstract：
A single strain from the family Paenibacillaceae was isolated from the wall behind the Waste Hygiene Compartment aboard the International Space Station (ISS) in April 2018, as part of the Microbial Tracking mission series. This strain was identified as a gram-positive, rod-shaped, oxidase-positive, catalase-negative motile bacterium in the genus Cohnella, designated as F6_2S_P_1T. The 16S sequence of the F6_2S_P_1T strain places it in a clade with C. rhizosphaerae and C. ginsengisoli, which were originally isolated from plant tissue or rhizosphere environments. The closest 16S and gyrB matches to strain F6_2S_P_1T are to C. rhizosphaerae with 98.84 and 93.99% sequence similarity, while a core single-copy gene phylogeny from all publicly available Cohnella genomes places it as more closely related to C. ginsengisoli. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values to any described Cohnella species are <89 and <22%, respectively. The major fatty acids for strain F6_2S_P_1T are anteiso-C15:0 (51.7%), iso-C16:0 (23.1%), and iso-C15:0 (10.5%), and it is able to metabolize a wide range of carbon compounds. Given the results of the ANI and dDDH analyses, this ISS strain is a novel species within the genus Cohnella for which we propose the name Cohnella hashimotonis, with the type strain F6_2S_P_1T (=NRRL B-65657T and DSMZ 115098T). Because no closely related Cohnella genomes were available, this study generated the whole-genome sequences (WGSs) of the type strains for C. rhizosphaerae and C. ginsengisoli. Phylogenetic and pangenomic analysis reveals that F6_2S_P_1T, C. rhizosphaerae, and C. ginsengisoli, along with two uncharacterized Cohnella strains, possess a shared set of 332 gene clusters which are not shared with any other WGS of Cohnella species, and form a distinct clade branching off from C. nanjingensis. Functional traits were predicted for the genomes of strain F6_2S_P_1T and other members of this clade.
摘要：
作为微生物追踪任务系列的一部分，2018年4月，从国际空间站（ISS）上的废物卫生室后面的墙上分离出了一种来自Paenibacillaceae家族的菌株。该菌株被鉴定为革兰氏阳性，杆状,氧化酶阳性,Cohnella属的过氧化氢酶阴性活动细菌，指定为F6_2S_P_1T。F6_2S_P_1T菌株的16S序列将其放置在具有根茎的C.ginsengisoli的进化枝中，最初是从植物组织或根际环境中分离出来的。与菌株F6_2S_P_1T最接近的16S和gyrB匹配与根茎C。具有98.84和93.99％的序列相似性，而来自所有公开可用的Cohnella基因组的核心单拷贝基因系统发育将其与C.ginsengisoli更密切相关。任何描述的Cohnella物种的平均核苷酸同一性（ANI）和数字DNA-DNA杂交（dDDH）值<89和<22％，分别。菌株F6_2S_P_1T的主要脂肪酸为antiso-C15：0（51.7％），iso-C16:0(23.1%)，和iso-C15：0（10.5%），它能够代谢各种碳化合物。鉴于ANI和dDDH分析的结果，这种ISS菌株是Cohnella属中的一种新物种，我们为此提出命名为Cohnellahashimotonis，菌株F6_2S_P_1T(=NRRLB-65657T和DSMZ115098T)。因为没有紧密相关的Cohnella基因组，这项研究产生了根茎梭菌和甘生梭菌类型菌株的全基因组序列（WGSs）。系统发育和全基因组分析表明，F6_2S_P_1T，C.根茎，和C.ginsengisoli，以及两种未鉴定的Cohnella菌株，拥有一组共享的332个基因簇，这些基因簇不与任何其他Cohnella物种的WGS共享，并形成一个从南京C.分支出来的独特进化枝。对菌株F6_2S_P_1T和该进化枝的其他成员的基因组进行了功能性状预测。
