PDF(Pubmed)
Abstract：
Short bowel syndrome (SBS) is a severe, life-threatening condition and one of the leading causes of intestinal failure in children. Here we were interested in changes in muscle layers and especially in the myenteric plexus of the enteric nervous system (ENS) of the small bowel in the context of intestinal adaptation. Twelve rats underwent a massive resection of the small intestine to induce SBS. Sham laparotomy without small bowel transection was performed in 10 rats. Two weeks after surgery, the remaining jejunum and ileum were harvested and studied. Samples of human small bowel were obtained from patients who underwent resection of small bowel segments due to a medical indication. Morphological changes in the muscle layers and the expression of nestin, a marker for neuronal plasticity, were studied. Following SBS, muscle tissue increases significantly in both parts of the small bowel, i.e., jejunum and ileum. The leading pathophysiological mechanism of these changes is hypertrophy. Additionally, we observed an increased nestin expression in the myenteric plexus in the remaining bowel with SBS. Our human data also showed that in patients with SBS, the proportion of stem cells in the myenteric plexus had risen by more than twofold. Our findings suggest that the ENS is tightly connected to changes in intestinal muscle layers and is critically involved in the process of intestinal adaptation to SBS.
摘要：
短肠综合征(SBS)是一种严重的,危及生命的状况和儿童肠衰竭的主要原因之一。在这里，我们对肌肉层的变化感兴趣，尤其是在肠道适应的情况下小肠肠神经系统（ENS）的肌间丛。12只大鼠接受了小肠的大量切除以诱导SBS。在10只大鼠中进行了无小肠横切的假剖腹手术。手术两周后,收获并研究剩余的空肠和回肠。从由于医学适应症而接受小肠段切除术的患者获得人小肠样品。肌肉层的形态变化和巢蛋白的表达，神经元可塑性的标记，被研究过。在SBS之后,肌肉组织在小肠的两个部分都显著增加，即，空肠和回肠.这些变化的主要病理生理机制是肥大。此外,我们观察到Nestin在SBS的其余肠肌间神经丛表达增加。我们的人类数据还表明，在SBS患者中，干细胞在肌间神经丛中的比例增加了两倍多。我们的发现表明，ENS与肠道肌肉层的变化密切相关，并且与肠道适应SBS的过程密切相关。
