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Abstract：
Since the first studies, the mouse models have provided crucial support for the most important discoveries on NK cells, on their development, function, and circulation within normal and tumor tissues. Murine tumor models were initially set to study murine NK cells, then, ever more sophisticated human-in-mice models have been developed to investigate the behavior of human NK cells and minimize the interferences from the murine environment. This review presents an overview of the models that have been used along time to study NK cells, focusing on the most popular NOG and NSG models, which work as recipients for the preparation of human-in-mice tumor models, the study of transferred human NK cells, and the evaluation of various enhancers of human NK cell function, including cytokines and chimeric molecules. Finally, an overview of the next generation humanized mice is also provided along with a discussion on how traditional and innovative in-vivo and in-vitro approaches could be integrated to optimize effective pre-clinical studies.
摘要：
自从第一次研究以来，小鼠模型为NK细胞最重要的发现提供了重要的支持，关于他们的发展，函数,和正常组织和肿瘤组织内的循环。小鼠肿瘤模型最初用于研究小鼠NK细胞，然后,已经开发了更复杂的人对小鼠模型来研究人类NK细胞的行为并将来自小鼠环境的干扰降至最低。这篇综述概述了一直以来用于研究NK细胞的模型，专注于最受欢迎的NOG和NSG模型，作为人类小鼠肿瘤模型制备的接受者，转移的人类NK细胞的研究，以及人类NK细胞功能的各种增强剂的评估，包括细胞因子和嵌合分子。最后,还提供了下一代人源化小鼠的概述，以及如何整合传统和创新的体内和体外方法以优化有效的临床前研究的讨论。
