PDF(Pubmed)
Abstract：
GATA3 and Mammaglobin are often used in the clinic to identify metastases of mammary origin due to their robust and diffuse expression in mammary tissue. However, the expression of these markers has not been well characterized in tumors from African American women. The goal of this study was to characterize and evaluate the expression of GATA3 and mammaglobin in breast tumors from African American women and determine their association with clinicopathological outcomes including breast cancer subtypes. Tissue microarrays (TMAs) were constructed from well preserved, morphologically representative tumors in archived formalin-fixed, paraffin-embedded (FFPE) surgical blocks from 202 patients with primary invasive ductal carcinoma. Mammaglobin and GATA3 expression was assessed using immunohistochemistry (IHC). Univariate analysis was carried out to determine the association between expression of GATA3, mammaglobin and clinicopathological characteristics. Kaplan-Meier estimates of overall survival and disease-free survival were also plotted and a log-rank test performed to compare estimates among groups. GATA3 expression showed statistically significant association with lower grade (p<0.001), ER-positivity (p<0.001), PR-positivity (p<0.001), and the luminal subtype (p<0.001). Mammaglobin expression was also significantly associated with lower grade (p=0.031), ER-positivity (p=0.007), and PR-positivity (p=0.022). There was no association with recurrence-free or overall survival. Our results confirm that GATA3 and mammaglobin demonstrate expression predominantly in luminal breast cancers from African American women. Additional markers with improved specificity and sensitivity are warranted for triple negative breast tumors given the high prevalence in women of African descent.
摘要：
GATA3和乳腺球蛋白由于其在乳腺组织中的稳健和弥漫性表达而经常在临床上用于识别乳腺起源的转移。然而,这些标志物的表达在非洲裔美国女性肿瘤中尚未得到很好的表征.这项研究的目的是表征和评估非洲裔美国女性乳腺肿瘤中GATA3和乳腺球蛋白的表达，并确定它们与包括乳腺癌亚型在内的临床病理结果的关系。组织微阵列（TMAs）是由保存完好的，保存的福尔马林固定的形态学代表性肿瘤，来自202例原发性浸润性导管癌患者的石蜡包埋（FFPE）手术块。使用免疫组织化学(IHC)评估乳腺球蛋白和GATA3表达。进行单因素分析以确定GATA3，乳腺球蛋白的表达与临床病理特征之间的关联。还绘制了总生存期和无病生存期的Kaplan-Meier估计值，并进行了对数秩检验以比较各组之间的估计值。GATA3表达与低级别有统计学意义（p<0.001），ER阳性（p<0.001），PR阳性（p<0.001），和管腔亚型（p<0.001）。乳房珠蛋白的表达也与低等级显著相关(p=0.031),ER阳性（p=0.007），和PR阳性（p=0.022）。与无复发或总生存期无关。我们的结果证实，GATA3和乳腺球蛋白主要在非裔美国妇女的管腔乳腺癌中表达。鉴于非洲裔女性的高患病率，对于三阴性乳腺肿瘤，需要具有改善的特异性和敏感性的其他标志物。
