Abstract：
The COVID-19 pandemic, caused by the SARS-CoV-2 virus, has been one of the most severe outbreaks of respiratory illness in history. The clinical symptoms of COVID-19 may be similar to flu, although they can be life-threatening, particularly in the elderly and immunocompromised population. Together with nucleic acid detection, serological testing has been essential for the diagnosis of SARS-CoV-2 infection but has been critically important for studying the epidemiology, serosurveillance, and for vaccine research and development. Multiplexed immunoassay technologies have a particular advantage as they can simultaneously measure multiple analytes from a single sample. xMAP technology is a multiplex analysis platform that can measure up to 500 analytes at the same time from the same sample. It has been shown to be an important tool for studying immune response to the various SARS-CoV-2 antigens, as well as for measuring host protein biomarker levels as prognostic indicators of COVID-19. In this chapter, we describe several key studies where xMAP technology was used for multiplexed analysis of SARS-COV-2 antibody responses and host protein expression in COVID-19 patients.
摘要：
COVID-19大流行，由SARS-CoV-2病毒引起，是历史上最严重的呼吸道疾病爆发之一。COVID-19的临床症状可能与流感相似，尽管它们可能会危及生命，特别是在老年人和免疫功能低下的人群中。连同核酸检测,血清学检测对于SARS-CoV-2感染的诊断至关重要，但对于研究流行病学至关重要，血清监测,以及疫苗研发。多重免疫测定技术具有特别的优势,因为它们可以同时测量来自单个样品的多个分析物。xMAP技术是一种多重分析平台，可同时从同一样品中测量多达500种分析物。它已被证明是研究对各种SARS-CoV-2抗原的免疫反应的重要工具，以及测量宿主蛋白生物标志物水平作为COVID-19的预后指标。在这一章中,我们描述了几项关键研究,其中使用xMAP技术对COVID-19患者的SARS-COV-2抗体反应和宿主蛋白表达进行多重分析.
