PDF(Pubmed)
Abstract：
Multidrug resistance (Mdr) proteins are critical proteins for maintenance of drug resistance in fungi. Mdr1 has been extensively studied in Candida albicans; its role in other fungi is largely unknown. In this study, we identified a homologous protein of Mdr (AoMdr1) in the nematode-trapping (NT) fungus Arthrobotrys oligospora. It was found that the deletion of Aomdr1 resulted in a significant reduction in the number of hyphal septa and nuclei as well as increased sensitivity to fluconazole and resistance to hyperosmotic stress and SDS. The deletion of Aomdr1 also led to a remarkable increase in the numbers of traps and mycelial loops in the traps. Notably, AoMdr1 was able to regulate mycelial fusion under low-nutrient conditions, but not under nutrient-rich conditions. AoMdr1 was also involved in secondary metabolism, and its deletion caused an increase in arthrobotrisins (specific compounds produced by NT fungi). These results suggest that AoMdr1 plays a crucial role in the fluconazole resistance, mycelial fusion, conidiation, trap formation, and secondary metabolism of A. oligospora. Our study contributes to the understanding of the critical role of Mdr proteins in mycelial growth and the development of NT fungi.
摘要：
多药耐药（Mdr）蛋白是维持真菌耐药性的关键蛋白。Mdr1已在白色念珠菌中进行了广泛研究；它在其他真菌中的作用尚不清楚。在这项研究中,我们在线虫捕获（NT）真菌Arthrobotrys寡孢子中鉴定了Mdr（AoMdr1）的同源蛋白。发现Aomdr1的缺失导致菌丝间隔和细胞核的数量显着减少，并增加了对氟康唑的敏感性以及对高渗胁迫和SDS的抗性。Aomdr1的缺失也导致陷阱和陷阱中菌丝环的数量显着增加。值得注意的是,AoMdr1能够在低营养条件下调节菌丝体融合，但不是在营养丰富的条件下。AoMdr1还参与了次级代谢，它的缺失导致节理霉素（NT真菌产生的特定化合物）增加。这些结果表明，AoMdr1在氟康唑耐药性中起着至关重要的作用。菌丝体融合，分生孢子，陷阱形成,和寡孢菌的次生代谢。我们的研究有助于了解Mdr蛋白在菌丝体生长和NT真菌发育中的关键作用。
