PDF(Pubmed)
Abstract：
In this bioremediation study, the fungus Penicillium sp. isolated from kefir grains was evaluated for its resistance to copper in the culture medium. Penicillium sp. was cultivated in liquid medium prepared using 2% malt-agar at pH 7.0. Biomass of the fungus was significantly reduced, but only when 800 mg·L-1 of Cu(NO3)2 copper nitrate was used. The effect on radial growth of the fungus in experiments combining different pH values and the inorganic contaminant showed an inhibition of 73% at pH 4.0, 75% at pH 7.0 and 77% at pH 9.0 in liquid medium. Thus, even though the growth of Penicillium sp. could be inhibited with relatively high doses of copper nitrate, images obtained with scanning electron microscopy showed the preservation of fungal cell integrity. Therefore, it can be concluded that Penicillium sp. isolated from kefir grains can survive while performing bioremediation to minimize the negative effects of copper on the environment through biosorption.
摘要：
在这项生物修复研究中，真菌青霉。评估了从开菲尔谷物中分离出的对培养基中铜的抗性。青霉。在使用2%麦芽琼脂在pH7.0制备的液体培养基中培养。真菌的生物量显著减少，但仅当使用800mg·L-1的Cu（NO3）2硝酸铜时。在结合不同pH值和无机污染物的实验中，对真菌径向生长的影响显示，在液体培养基中，在pH4.0时为73％，在pH7.0时为75％，在pH9.0时为77％。因此，即使青霉的生长。可以用相对高剂量的硝酸铜抑制，扫描电子显微镜获得的图像显示真菌细胞的完整性保存。因此,可以得出结论，青霉菌。从开菲尔谷物中分离出来的谷物可以在进行生物修复时存活，以通过生物吸附将铜对环境的负面影响降至最低。
