PDF(Pubmed)
Abstract：
From acute to chronic hepatitis, cirrhosis, and hepatocellular cancer, hepatitis B infection causes a broad spectrum of liver diseases. Molecular and serological tests have been used to diagnose hepatitis B-related illnesses. Due to technology limitations, it is challenging to identify hepatitis B infection cases at an early stage, particularly in a low- and middle-income country with constrained resources. Generally, the gold-standard methods to detect hepatitis B virus (HBV) infection requires dedicated personnel, bulky, expensive equipment and reagents, and long processing times which delay the diagnosis of HBV. Thus, lateral flow assay (LFA), which is inexpensive, straightforward, portable, and operates reliably, has dominated point-of-care diagnostics. LFA consists of four parts: a sample pad where samples are dropped; a conjugate pad where labeled tags and biomarker components are combined; a nitrocellulose membrane with test and control lines for target DNA-probe DNA hybridization or antigen-antibody interaction; and a wicking pad where waste is stored. By modifying the pre-treatment during the sample preparation process or enhancing the signal of the biomarker probes on the membrane pad, the accuracy of the LFA for qualitative and quantitative analysis can be improved. In this review, we assembled the most recent developments in LFA technologies for the progress of hepatitis B infection detection. Prospects for ongoing development in this area are also covered.
摘要：
从急性到慢性肝炎，肝硬化,和肝细胞癌，乙型肝炎感染引起广泛的肝脏疾病。分子和血清学测试已用于诊断乙型肝炎相关疾病。由于技术限制,在早期阶段识别乙型肝炎感染病例是具有挑战性的，特别是在资源有限的中低收入国家。一般来说,检测乙型肝炎病毒（HBV）感染的金标准方法需要专门的人员，笨重,昂贵的设备和试剂，和长的处理时间延迟HBV的诊断。因此，侧流测定（LFA），它很便宜，直截了当，便携式，并且运行可靠，主导了即时诊断。LFA由四个部分组成:样品垫,其中样品被滴下;缀合垫,其中标记的标签和生物标志物组分被组合;硝酸纤维素膜,其具有用于靶DNA-探针DNA杂交或抗原-抗体相互作用的测试线和对照线;以及芯吸垫,其中废物被存储。通过在样品制备过程中修改预处理或增强膜垫上的生物标志物探针的信号，可以提高LFA定性和定量分析的准确性。在这次审查中,我们汇集了LFA技术的最新进展，用于乙型肝炎感染检测的进展。还涵盖了该领域正在进行的发展前景。
