PDF(Pubmed)
Abstract：
The rising prevalence of antibiotic-resistance is currently a grave issue; hence, novel antimicrobial agents are being explored and developed to address infections resulting from multiple drug-resistant pathogens. Biogenic CuO, ZnO, and WO3 nanoparticles can be considered as such agents. Clinical isolates of E. coli, S. aureus, methicillin-resistant S. aureus (MRSA), and Candida albicans from oral and vaginal samples were treated with single and combination metal nanoparticles incubated under dark and light conditions to understand the synergistic effect of the nanoparticles and their photocatalytic antimicrobial activity. Biogenic CuO and ZnO nanoparticles exhibited significant antimicrobial effects under dark incubation which did not alter on photoactivation. However, photoactivated WO3 nanoparticles significantly reduced the number of viable cells by 75% for all the test organisms, thus proving to be a promising antimicrobial agent. Combinations of CuO, ZnO, and WO3 nanoparticles demonstrated synergistic action as a significant increase in their antimicrobial property (>90%) was observed compared to the action of single elemental nanoparticles. The mechanism of the antimicrobial action of metal nanoparticles both in combination and in isolation was assessed with respect to lipid peroxidation due to ROS (reactive oxygen species) generation by measuring malondialdehyde (MDA) production, and the damage to cell integrity using live/dead staining and quantitating with the use of flow cytometry and fluorescence microscopy.
摘要：
抗生素耐药性的流行率上升是目前一个严重的问题；因此，正在探索和开发新型抗菌药物，以解决由多种耐药病原体引起的感染。生物CuO,ZnO,和WO3纳米颗粒可以被认为是这样的试剂。大肠杆菌临床分离株，金黄色葡萄球菌，耐甲氧西林金黄色葡萄球菌（MRSA），将口腔和阴道样品中的白色念珠菌和白色念珠菌用在黑暗和光照条件下孵育的单一和组合金属纳米颗粒处理，以了解纳米颗粒的协同作用及其光催化抗菌活性。生物CuO和ZnO纳米颗粒在黑暗孵育下表现出显着的抗微生物作用，而光活化不会改变。然而,光活化的WO3纳米粒子显着减少了所有测试生物的活细胞数量的75％，因此被证明是一种有前途的抗菌剂。CuO的组合，ZnO,和WO3纳米颗粒表现出协同作用,因为与单一元素纳米颗粒的作用相比,观察到它们的抗微生物性质显著增加(>90%)。通过测量丙二醛（MDA）的产生，评估了由于ROS（活性氧）产生引起的脂质过氧化作用，金属纳米颗粒在组合和分离中的抗菌作用机理。以及使用活/死染色和使用流式细胞术和荧光显微镜定量对细胞完整性的损害。
