PDF(Pubmed)
Abstract：
Muscle cell growth plays an important role in skeletal muscle development. Circular RNAs (circRNAs) have been proven to be involved in the regulation of skeletal muscle growth and development. In this study, we explored the effect of circTTN on myoblast growth and its possible molecular mechanism. Using C2C12 cells as a functional model, the authenticity of circTTN was confirmed by RNase R digestion and Sanger sequencing. Previous functional studies have showed that the overexpression of circTTN inhibits myoblast proliferation and differentiation. Mechanistically, circTTN recruits the PURB protein on the Titin (TTN) promoter to inhibit the expression of the TTN gene. Moreover, PURB inhibits myoblast proliferation and differentiation, which is consistent with circTTN function. In summary, our results indicate that circTTN inhibits the transcription and myogenesis of the host gene TTN by recruiting PURB proteins to form heterotypic complexes. This work may act as a reference for further research on the role of circRNA in skeletal muscle growth and development.
摘要：
肌细胞生长在骨骼肌发育中起重要作用。环状RNA（circularRNAs）已被证明参与骨骼肌生长和发育的调节。在这项研究中,我们探讨了circTTN对成肌细胞生长的影响及其可能的分子机制。使用C2C12细胞作为功能模型，通过RNaseR消化和Sanger测序证实了circTTN的真实性。先前的功能研究表明，circtTN的过表达抑制成肌细胞的增殖和分化。机械上,circTTN在Titin（TTN）启动子上募集PURB蛋白以抑制TTN基因的表达。此外，PURB抑制成肌细胞增殖和分化，这与circTTN函数一致。总之,我们的结果表明，circtTN通过募集PURB蛋白形成异型复合物来抑制宿主基因TTN的转录和成肌。这项工作可能为进一步研究circRNA在骨骼肌生长发育中的作用提供参考。
