PDF(Pubmed)
Abstract：
It has been well documented that an infestation of the piercing-sucking herbivore, brown planthopper (BPH), Nilaparvata lugens, activates strong local defenses in rice. However, whether a BPH infestation elicits systemic responses in rice remains largely unknown. In this study, we investigated BPH-induced systemic defenses by detecting the change in expression levels of 12 JA- and/or SA-signaling-responsive marker genes in different rice tissues upon a BPH attack. We found that an infestation of gravid BPH females on rice leaf sheaths significantly increased the local transcript level of all 12 marker genes tested except OsVSP, whose expression was induced only weakly at a later stage of the BPH infestation. Moreover, an infestation of gravid BPH females also systemically up-regulated the transcription levels of three JA-signaling-responsive genes (OsJAZ8, OsJAMyb, and OsPR3), one SA-signaling-responsive gene (OsWRKY62), and two JA- and SA- signaling-responsive genes (OsPR1a and OsPR10a). Our results demonstrate that an infestation of gravid BPH females systemically activates JA- and SA-dependent defenses in rice, which may in turn influence the composition and structure of the community in the rice ecosystem.
摘要：
有充分的证据表明，刺吸食草动物的侵扰，褐飞虱(BPH),Nilaparvatalugens,激活稻米的强大局部防御。然而,BPH侵染是否在水稻中引起系统反应仍在很大程度上未知。在这项研究中,我们通过检测BPH攻击后不同水稻组织中12个JAA和/或SA信号应答标记基因表达水平的变化，研究了BPH诱导的全身防御。我们发现，受孕BPH雌性在水稻叶鞘上的侵染显着增加了除OsVSP外的所有12个标记基因的局部转录水平，在BPH感染的后期仅微弱地诱导其表达。此外，妊娠BPH雌性的侵染也系统地上调了三个JA信号反应基因的转录水平（OsJAZ8，OsJAMyb，和OsPR3），一个SA信号传导响应基因（OsWRKY62），和两个JAA-和SA-信号传导响应基因(OsPR1a和OsPR10a)。我们的结果表明，妊娠BPH雌性的侵染会系统地激活水稻中的JAA和SA依赖性防御，进而影响水稻生态系统中群落的组成和结构。
