PDF(Pubmed)
Abstract：
Tamarindus indica Linn (tamarind, F. Leguminosae) is one of the most widely consumed edible fruits in the world. Phytochemical investigation of tamarind pulp n-butanol fraction yielded one new (+)-pinitol glycoside compound 1 (25% w/w), and 1D, 2D NMR, and HRESIMS investigation were used to confirm the new compound\'s structure. (+)-Pinitol glycoside showed anti-Alzheimer potential that was confirmed in prophylactic and treatment groups by decreasing time for the T-maze test; decreased TAO, brain and serum AChE, MDA, tau protein levels, and β amyloid peptide protein levels; and increasing GPX, SOD levels, and in vivo regression of the neurodegenerative features of Alzheimer\'s dementia in an aluminum-intoxicated rat model. The reported molecular targets for human Alzheimer\'s disease were then used in a network pharmacology investigation to examine their complex interactions and identify the key targets in the disease pathogenesis. An in silico-based analysis (molecular docking, binding free energy calculation (ΔGBinding), and molecular dynamics simulation) was performed to identify the potential targets for compound 1. The findings of this study may lead to the development of dietary supplements for the treatment of Alzheimer\'s disease.
摘要：
罗望子（罗望子，F.豆科）是世界上食用最广泛的可食用水果之一。罗望子纸浆正丁醇部分的植物化学研究产生了一种新的（）-松醇糖苷化合物1（25％w/w），1D,2DNMR,和HRESIMS研究用于确认新化合物的结构。（+）-Pinitol糖苷显示抗阿尔茨海默病潜力，这在预防和治疗组中通过减少T-迷宫测试的时间得到证实；TAO减少，大脑和血清AChE，MDA,tau蛋白水平,和β淀粉样肽蛋白水平;和增加GPX，SOD水平，在铝中毒大鼠模型中，阿尔茨海默氏痴呆的神经退行性特征在体内消退。然后将报道的人类阿尔茨海默病的分子靶标用于网络药理学研究中，以检查它们的复杂相互作用并确定疾病发病机理中的关键靶标。基于硅的分析(分子对接，结合自由能计算(ΔGBinding)，和分子动力学模拟）进行，以确定化合物1的潜在靶标。这项研究的发现可能会导致膳食补充剂的发展用于治疗阿尔茨海默病。
