PDF(Pubmed)
Abstract：
Ganpu vine tea is a new type of health care citrus fruit tea made from citrus shell, Pu-er tea, and vine tea baked as raw materials. In this study, the in vitro uric acid synthase inhibition system and hyperuric acid cell model were constructed to appraise the uric acid lowering efficacy of Ganpu vine tea, traditional Ganpu tea, and vine tea. Results showed that in the uric acid synthase inhibition system, the aqueous extract can inhibite the puric metabolically related enzymes, such as adenosine deaminase (ADA), purine nucleoside phosphorylase (PNP), and xanthine oxidase (XOD). The ability of the aqueous extract to inhibit the above enzyme was as follows: vine tea > Ganpu vine tea > Ganpu tea; all teas had a strong effect on XOD inhibition. The hyperuric acid cell model test showed that the aqueous extract inhibited uric acid production through accumulating inosine and hypoxanthine and hindering xanthine synthesis. The uric acid reductive ability was as follows: Vine tea > Ganpu vine tea > Ganpu tea. The inhibition of enzymes related to uric acid synthesis and the inhibition of uric acid production were significantly enhanced through adding vine tea to Ganpu tea. It also shows that flavonoids are the main factor driving this ability because they are the main active ingredients in these botanical drinks.
摘要：
赣浦藤茶是一种以柑橘壳为原料制成的新型保健柑橘类水果茶，普洱茶,和藤茶烘烤为原料。在这项研究中,建立了体外尿酸合成酶抑制系统和高尿酸细胞模型，评价了甘朴藤茶的降尿酸功效，传统的甘浦茶，和葡萄树茶。结果表明,在尿酸合成酶抑制体系中,水提取物可以抑制嘌呤代谢相关酶，如腺苷脱氨酶（ADA），嘌呤核苷磷酸化酶（PNP），和黄嘌呤氧化酶(XOD)。水提物对上述酶的抑制能力如下:藤茶>甘浦藤茶>甘浦茶;所有茶对XOD的抑制作用都很强。高尿酸细胞模型试验表明，水提物通过积累肌苷和次黄嘌呤并阻碍黄嘌呤的合成来抑制尿酸的产生。尿酸还原能力如下：藤茶>甘浦藤茶>甘浦茶。通过在甘朴茶中添加藤茶，对尿酸合成相关酶的抑制作用和尿酸生产的抑制作用明显增强。它还表明，类黄酮是驱动这种能力的主要因素，因为它们是这些植物饮料中的主要活性成分。
