Abstract：
This study investigated anti-melanogenesis effects of enzyme-treated caviar extract (CV) in murine melanoma B16F10 cells and SKH-1 hairless mice. To induce melanin production in vitro and in vivo studies, B16F10 cells were treated with 3-Isobutyl-1-methylxanthine (IBMX), and SKH-1 hairless mice were irradiated with UVB, respectively. The expression of melnogenesis-related factors and signaling molecules were analyzed by ELISA and western blotting. 50, 100 and 200 μg/mL of CV significantly decreased the melanin contents and the activities of tyrosinase, nitric oxide, glutathione, and cAMP, melanogenesis factor, in B16F10 cells treated IBMX. In addition, CV significantly suppressed the expression of melanogenesis proteins such as pPKA, pCREB, MITF, TRP-1and TRP-2. Similarly, results of oral administration of CV (20, 50 and 100 mg/kg) for 8 weeks in UVB-Induced SKH-1 hairless mice, the expression of melanogenesis-related factor tyrosinase, nitric oxide, and cAMP and protein expression of pPKA, pCREBa, MITF, TRP-1and TRP-2 was significantly reduced. In particular, 100 mg/kg of CV exhibited an excellent effect similar to control group. Therefore, we suggest the possibility of developing CV as a food supplement having skin whitening effects by ameliorating melanogenesis.
摘要：
这项研究研究了酶处理的鱼子酱提取物（CV）在小鼠黑色素瘤B16F10细胞和SKH-1无毛小鼠中的抗黑素生成作用。诱导黑色素产生的体外和体内研究，B16F10细胞用3-异丁基-1-甲基黄嘌呤（IBMX）处理，用UVB照射SKH-1无毛小鼠，分别。通过ELISA和蛋白质印迹分析黑化发生相关因子和信号分子的表达。50、100和200μg/mL的CV显著降低黑色素含量和酪氨酸酶活性,一氧化氮,谷胱甘肽,和cAMP，黑色素生成因子，在B16F10细胞处理的IBMX中。此外,CV显著抑制黑素生成蛋白如pPKA的表达，pCREB,MITF,TRP-1和TRP-2。同样，在UVB诱导的SKH-1无毛小鼠中口服CV（20、50和100mg/kg）8周的结果，黑素生成相关因子酪氨酸酶的表达,一氧化氮,cAMP和pPKA蛋白的表达,pCREBa,MITF,TRP-1和TRP-2显著降低。特别是,100mg/kg的CV表现出与对照组相似的优异效果。因此,我们建议通过改善黑色素生成来开发CV作为具有皮肤美白作用的食品补充剂的可能性。
