PDF(Pubmed)
Abstract：
UNASSIGNED: The stability of the analytes most commonly used in routine clinical practice has been the subject of intensive research, with varying and even conflicting results. Such is the case of alanine aminotransferase (ALT). The purpose of this study was to determine the stability of serum ALT according to different variables.
UNASSIGNED: A multicentric study was conducted in eight laboratories using serum samples with known initial catalytic concentrations of ALT within four different ranges, namely: <50 U/L (<0.83 μkat/L), 50-200 U/L (0.83-3.33 μkat/L), 200-400 U/L (3.33-6.67 μkat/L) and >400 U/L (>6.67 μkat/L). Samples were stored for seven days at two different temperatures using four experimental models and four laboratory analytical platforms. The respective stability equations were calculated by linear regression. A multivariate model was used to assess the influence of different variables.
UNASSIGNED: Catalytic concentrations of ALT decreased gradually over time. Temperature (-4%/day at room temperature vs. -1%/day under refrigeration) and the analytical platform had a significant impact, with Architect (Abbott) showing the greatest instability. Initial catalytic concentrations of ALT only had a slight impact on stability, whereas the experimental model had no impact at all.
UNASSIGNED: The constant decrease in serum ALT is reduced when refrigerated. Scarcely studied variables were found to have a significant impact on ALT stability. This observation, added to a considerable inter-individual variability, makes larger studies necessary for the definition of stability equations.
UNASSIGNED: A multicentric study was conducted in eight laboratories using serum samples with known initial catalytic concentrations of ALT within four different ranges, namely: <50 U/L (<0.83 μkat/L), 50-200 U/L (0.83-3.33 μkat/L), 200-400 U/L (3.33-6.67 μkat/L) and >400 U/L (>6.67 μkat/L). Samples were stored for seven days at two different temperatures using four experimental models and four laboratory analytical platforms. The respective stability equations were calculated by linear regression. A multivariate model was used to assess the influence of different variables.
UNASSIGNED: Catalytic concentrations of ALT decreased gradually over time. Temperature (-4%/day at room temperature vs. -1%/day under refrigeration) and the analytical platform had a significant impact, with Architect (Abbott) showing the greatest instability. Initial catalytic concentrations of ALT only had a slight impact on stability, whereas the experimental model had no impact at all.
UNASSIGNED: The constant decrease in serum ALT is reduced when refrigerated. Scarcely studied variables were found to have a significant impact on ALT stability. This observation, added to a considerable inter-individual variability, makes larger studies necessary for the definition of stability equations.
摘要：
■常规临床实践中最常用的分析物的稳定性一直是深入研究的主题，结果各不相同，甚至相互矛盾。丙氨酸转氨酶(ALT)就是这种情况。本研究的目的是根据不同变量确定血清ALT的稳定性。
■在八个实验室中进行了一项多中心研究，使用已知ALT初始催化浓度在四个不同范围内的血清样品，即：<50U/L（<0.83μkat/L），50-200U/L(0.83-3.33μkat/L),200-400U/L（3.33-6.67μkat/L）和>400U/L（>6.67μkat/L）。使用四个实验模型和四个实验室分析平台将样品在两种不同的温度下储存七天。通过线性回归计算各自的稳定性方程。使用多变量模型来评估不同变量的影响。
■ALT的催化浓度随时间逐渐降低。温度（室温下-4％/天与-1％/天的冷藏）和分析平台产生了重大影响，建筑师(雅培)表现出最大的不稳定性。ALT的初始催化浓度仅对稳定性有轻微影响，而实验模型完全没有影响.
■当冷藏时,血清ALT的恒定降低降低。几乎没有研究的变量被发现对ALT稳定性有显著影响。这个观察,增加了相当大的个体间变异性，对稳定性方程的定义进行了更大的研究。
■在八个实验室中进行了一项多中心研究，使用已知ALT初始催化浓度在四个不同范围内的血清样品，即：<50U/L（<0.83μkat/L），50-200U/L(0.83-3.33μkat/L),200-400U/L（3.33-6.67μkat/L）和>400U/L（>6.67μkat/L）。使用四个实验模型和四个实验室分析平台将样品在两种不同的温度下储存七天。通过线性回归计算各自的稳定性方程。使用多变量模型来评估不同变量的影响。
■ALT的催化浓度随时间逐渐降低。温度（室温下-4％/天与-1％/天的冷藏）和分析平台产生了重大影响，建筑师(雅培)表现出最大的不稳定性。ALT的初始催化浓度仅对稳定性有轻微影响，而实验模型完全没有影响.
■当冷藏时,血清ALT的恒定降低降低。几乎没有研究的变量被发现对ALT稳定性有显著影响。这个观察,增加了相当大的个体间变异性，对稳定性方程的定义进行了更大的研究。
