PDF(Pubmed)
Abstract：
Interferences in the clinical laboratory may lead physicians misinterpret results for some biological analytes. The most common analytical interferences in the clinical laboratory include hemolysis, icterus and lipemia. Lipemia is defined as turbidity in a sample caused by the accumulation of lipoproteins, mainly very-low density lipoproteins (VLDL) and chylomicrons. Several methods are available for the detection of lipemic samples, including the lipemic index, or triglyceride quantification in serum or plasma samples, or mean corpuscular hemoglobin (MCHC) concentration in blood samples. According to the European Directive 98/79/CE, it is the responsibility of clinical laboratories to monitor the presence of interfering substances that may affect the measurement of an analyte. There is an urgent need to standardize interference studies and the way interferences are reported by manufacturers. Several methods are currently available to remove interference from lipemia and enable accurate measurement of biological quantities. The clinical laboratory should establish a protocol for the handling of lipemic samples according to the biological quantity to be tested.
摘要：
临床实验室中的干扰可能导致医生误解某些生物分析物的结果。临床实验室中最常见的分析干扰包括溶血，黄疸和脂血.血脂被定义为由脂蛋白的积累引起的样品中的浊度，主要是极低密度脂蛋白（VLDL）和乳糜微粒。有几种方法可用于检测血脂样本，包括血脂指数，或血清或血浆样品中的甘油三酯定量，或血样中平均红细胞血红蛋白（MCHC）浓度。根据欧洲指令98/79/CE，临床实验室负责监测可能影响分析物测量的干扰物质的存在。迫切需要标准化干扰研究以及制造商报告干扰的方式。目前有几种方法可用于去除脂血的干扰并能够精确测量生物量。临床实验室应根据要测试的生物量建立处理脂血样品的方案。
