Abstract：
Daily, people are exposed to chemicals and environmental compounds such as bisphenols (BPs). These substances are present in more than 80% of human fluids. Human exposure to BPs is associated with male reproductive health disorders. Some of the main targets of BPs are intercellular junction proteins of the blood-testis barrier (BTB) in Sertoli cells because BPs alter the expression or induce aberrant localization of these proteins. In this systematic review, we explore the effects of BP exposure on the expression of BTB junction proteins and the characteristics of in vivo studies to identify potential gaps and priorities for future research. To this end, we conducted a systematic review of articles. Thirteen studies met our inclusion criteria. In most studies, animals treated with bisphenol-A (BPA) showed decreased occludin expression at all tested doses. However, bisphenol-AF treatment did not alter occludin expression. Cx43, ZO-1, β-catenin, nectin-3, cortactin, paladin, and claudin-11 expression also decreased in some tested doses of BP, while N-cadherin and FAK expression increased. BP treatment did not alter the expression of α and γ catenin, E-cadherin, JAM-A, and Arp 3. However, the expression of all these proteins was altered when BPA was administered to neonatal rodents in microgram doses. The results show significant heterogeneity between studies. Thus, it is necessary to perform more research to characterize the changes in BTB protein expression induced by BPs in animals to highlight future research directions that can inform the evaluation of risk of toxicity in humans.
摘要：
每日,人们暴露于化学物质和环境化合物，如双酚（BPs）。这些物质存在于80%以上的人体液体中。人类暴露于BP与男性生殖健康障碍有关。BP的一些主要靶标是支持细胞中血液睾丸屏障（BTB）的细胞间连接蛋白，因为BP会改变这些蛋白的表达或诱导这些蛋白的异常定位。在这次系统审查中，我们探讨了BP暴露对BTB连接蛋白表达的影响以及体内研究的特点,以确定潜在的差距和未来研究的重点.为此,我们对文章进行了系统的回顾。13项研究符合我们的纳入标准。在大多数研究中,用双酚A（BPA）处理的动物在所有测试剂量下显示出降低的闭塞蛋白表达。然而,双酚AF处理没有改变闭塞蛋白的表达。Cx43，ZO-1，β-连环蛋白，nectin-3,cortactin,圣骑士,claudin-11的表达在一些测试剂量的BP中也降低，而N-cadherin和FAK表达增加。BP治疗没有改变α和γcatenin的表达，E-cadherin,JAM-A,Arp3然而,当BPA以微克剂量给予新生啮齿动物时,所有这些蛋白的表达都发生了改变.结果显示研究之间存在显著的异质性。因此，有必要进行更多的研究来表征BPs在动物中诱导的BTB蛋白表达的变化，以突出未来的研究方向，可以为评估人类毒性风险提供信息。
