Abstract：
Au coated magnetic polyphosphazene (MPCTP) composite particles (MPCTP@Au) were fabricated with sensitive SERS activity. The MPCTP particles were generated by coating polyphosphazene on Fe3O4 nanoparticles through precipitation polycondensation of hexachlorocyclotriphosphazene and phloroglucinol. MPCTP@Au composite particles were obtained by deposition of Au nanoparticles on MPCTP by the reduction of HAuCl4. The size and the thickness of the Au shell can be controlled by varying the amount of HAuCl4. The magnetic core endowed the composite particles with good magnetic responsiveness, which allowed the analyte to be enriched and separated from the complex matrix, and significantly simplifying the sample pretreatment procedure. The SERS activity of MPCTP@Au composite particles were evaluated by DTNB as model Raman reporter, and the limits of detection (LOD) of DTNB was 10-8 mol/L. A high efficient SERS immunoassay system based on the MPCTP@Au substrates for the detection of immunoproteins was developed. Human IgG and rabbit IgG were quantitatively determinated simultaneously by this immunoassay system. The quantitative determination of the immunoglobulin G (IgG) was achieved and the LOD of human IgG, rabbit IgG and the mixture of human IgG and rabbit IgG were as low as 10 fg/mL, 100 pg/mL and 1 ng/mL, respectively. The results showed that the MPCTP@Au composite particles have broad application prospects as high performance SERS active substrates for immunoprotein analysis.
摘要：
制备了具有敏感SERS活性的Au包覆磁性聚磷腈（MPCTP）复合颗粒（MPCTP@Au）。MPCTP颗粒是通过六氯环三磷腈和间苯三酚的沉淀缩聚将聚磷腈包覆在Fe3O4纳米颗粒上而生成的。通过还原HAuCl4在MPCTP上沉积Au纳米颗粒获得MPCTP@Au复合颗粒。Au壳的尺寸和厚度可以通过改变HAuCl4的量来控制。磁芯赋予复合粒子良好的磁响应性,这使得分析物可以从复杂的基质中富集和分离，大大简化了样品预处理程序。以DTNB为模型拉曼报道分子,评价了MPCTP@Au复合颗粒的SERS活性,DTNB的检出限（LOD）为10-8mol/L。开发了一种基于MPCTP@Au底物的高效SERS免疫测定系统，用于检测免疫蛋白。通过该免疫测定系统同时定量测定人IgG和兔IgG。免疫球蛋白G(IgG)的定量测定和人IgG的LOD,兔IgG和人IgG和兔IgG的混合物低至10fg/mL，100pg/mL和1ng/mL，分别。结果表明，MPCTP@Au复合颗粒作为免疫蛋白分析的高性能SERS活性底物具有广阔的应用前景。
