Abstract：
OBJECTIVE: Study the possible mechanism and delayed effect of tilapia skin collagen on skin aging for mice.
METHODS: Kunming (KM) mice were randomly divided into the aging model group, the normal group, the positive control group (vitamin E) and the low, medium, high dose tilapia skin collagen groups (2.0, 4.0, 8.0 mg/g). The normal group was only injected with saline at the back and the neck. The other groups were injected subcutaneously with 5% D-galactose and ultraviolet light jointly to establish the aging model. After modeling, the positive control group was treated with a dose of 10% vitamin E once a day, and the low, medium, high dose tilapia skin collagen group was separately applied 2.0, 4.0, 8.0 mg/g of tilapia skin collagen for 40 days. The changes of skin tissue morphology, water content, hydroxyproline (Hyp) content, and superoxide dismutase (SOD) activity in mice were studied at the day 10, 20, 30, 40, 50.
RESULTS: Compared with the normal group, the skin of mice in the aging model group was thinner, looser, and the skin moisture content, Hyp content, SOD activity were all decreased. For mice of the low, medium, high dose tilapia skin collagen group, the thickness of dermis increased, possessing close arrangement, and the moisture content, Hyp content, SOD activity were up-regulated significantly, which effectively alleviated the aging process of skin. The dose of tilapia skin collagen was directly proportional to the anti-aging effect.
CONCLUSIONS: Tilapia skin collagen has an obvious effect on improving skin aging.
METHODS: Kunming (KM) mice were randomly divided into the aging model group, the normal group, the positive control group (vitamin E) and the low, medium, high dose tilapia skin collagen groups (2.0, 4.0, 8.0 mg/g). The normal group was only injected with saline at the back and the neck. The other groups were injected subcutaneously with 5% D-galactose and ultraviolet light jointly to establish the aging model. After modeling, the positive control group was treated with a dose of 10% vitamin E once a day, and the low, medium, high dose tilapia skin collagen group was separately applied 2.0, 4.0, 8.0 mg/g of tilapia skin collagen for 40 days. The changes of skin tissue morphology, water content, hydroxyproline (Hyp) content, and superoxide dismutase (SOD) activity in mice were studied at the day 10, 20, 30, 40, 50.
RESULTS: Compared with the normal group, the skin of mice in the aging model group was thinner, looser, and the skin moisture content, Hyp content, SOD activity were all decreased. For mice of the low, medium, high dose tilapia skin collagen group, the thickness of dermis increased, possessing close arrangement, and the moisture content, Hyp content, SOD activity were up-regulated significantly, which effectively alleviated the aging process of skin. The dose of tilapia skin collagen was directly proportional to the anti-aging effect.
CONCLUSIONS: Tilapia skin collagen has an obvious effect on improving skin aging.
摘要：
目的：研究罗非鱼皮肤胶原蛋白对小鼠皮肤老化的可能机制及延缓作用。
方法：昆明种(KM)小鼠随机分为衰老模型组,正常组,阳性对照组（维生素E）和低，中等,高剂量罗非鱼皮肤胶原蛋白组（2.0、4.0、8.0mg/g）。正常组仅在背部和颈部注射生理盐水。其余各组分别皮下注射5%D-半乳糖和紫外光联合建立衰老模型。建模后,阳性对照组每日1次服用10%的维生素E,和低，中等,高剂量罗非鱼皮胶原蛋白组分别施用2.0、4.0、8.0mg/g罗非鱼皮胶原蛋白40天。皮肤组织形态的变化,含水量,羟脯氨酸(Hyp)含量,在第10、20、30、40、50天研究小鼠的超氧化物歧化酶（SOD）活性。
结果：与正常组相比，衰老模型组小鼠皮肤变薄，更宽松，和皮肤水分含量，Hyp含量,SOD活性均下降。对于低的老鼠来说，中等,高剂量罗非鱼皮胶原蛋白组，真皮厚度增加，拥有紧密的安排，和水分含量，Hyp含量,SOD活性显著上调,有效缓解皮肤老化过程。罗非鱼皮胶原蛋白的剂量与抗衰老效果成正比。
结论：罗非鱼皮胶原蛋白有明显的改善皮肤老化的作用。
方法：昆明种(KM)小鼠随机分为衰老模型组,正常组,阳性对照组（维生素E）和低，中等,高剂量罗非鱼皮肤胶原蛋白组（2.0、4.0、8.0mg/g）。正常组仅在背部和颈部注射生理盐水。其余各组分别皮下注射5%D-半乳糖和紫外光联合建立衰老模型。建模后,阳性对照组每日1次服用10%的维生素E,和低，中等,高剂量罗非鱼皮胶原蛋白组分别施用2.0、4.0、8.0mg/g罗非鱼皮胶原蛋白40天。皮肤组织形态的变化,含水量,羟脯氨酸(Hyp)含量,在第10、20、30、40、50天研究小鼠的超氧化物歧化酶（SOD）活性。
结果：与正常组相比，衰老模型组小鼠皮肤变薄，更宽松，和皮肤水分含量，Hyp含量,SOD活性均下降。对于低的老鼠来说，中等,高剂量罗非鱼皮胶原蛋白组，真皮厚度增加，拥有紧密的安排，和水分含量，Hyp含量,SOD活性显著上调,有效缓解皮肤老化过程。罗非鱼皮胶原蛋白的剂量与抗衰老效果成正比。
结论：罗非鱼皮胶原蛋白有明显的改善皮肤老化的作用。
