PDF(Pubmed)
Abstract：
The mandate of the current investigation was to elucidate the therapeutic and antioxidant perspective of black tea. Purposely, black tea compositional analysis followed by polyphenol extraction and antioxidant characterization was done. Moreover, the theaflavin from black tea extract was also isolated through the solvent partition method. Lastly, the neuroprotective effect of isolated theaflavin was assessed through a bio-efficacy trial. The outcomes delineated that black tea showed promising nutritional composition with special reference to protein and fiber. Among the extraction solvent, ethanol performed better as compared to methanol and water likewise, higher extraction was noticed at 60 min followed by 90 and 30 min. All the extracts indicated antioxidant activity reflected through significant DPPH, TPC, FRAP, and beta carotene as-69.13 ± 3.00, 1148.92 ± 14.01, 752.44 ± 10.30, and 65.74 ± 3.28, respectively. However, isolated theaflavin exhibited higher antioxidant capacity as-737.74 ± 12.55, 82.60 ± 2.33, and 853.77 ± 9.55, for TPC, DPPH, and FRAP, respectively, as compared to extracts. In 15 days\' efficacy was physically induced with sciatic nerve injury h sciatic nerve injury physically and treated with isolated theaflavin. A total of 12 healthy albino mice were randomly assigned to either the control (n = 6) or theaflavin (5.0 mg/kg (n = 6)) groups. In these groups, behavioral tests were used to assess and compare enhanced functional recovery as well as skeletal muscle mass measurement. Serum samples included oxidative stress markers. In theaflavin leaves, behavioral tests revealed a statistically significant (p < .001) improvement in sensorimotor function restoration, muscle mass restoration, a substantial decrease in TOS, a significant increase in TAC, and enhanced antioxidative enzyme activity. Considering the above-mentioned therapeutic perspectives of theaflavin, the current research was planned to optimize the isolation of theaflavin from black tea and probed for their neuroprotective effect in mice models.
摘要：
当前调查的任务是阐明红茶的治疗和抗氧化观点。故意,红茶成分分析，多酚提取和抗氧化表征。此外，还通过溶剂分配法从红茶提取物中分离出茶黄素。最后,通过一项生物功效试验评估了分离的茶黄素的神经保护作用.结果表明，红茶显示出有希望的营养成分，特别涉及蛋白质和纤维。在萃取溶剂中，与甲醇和水相比，乙醇的性能更好，在60分钟后90分钟和30分钟观察到更高的提取。所有提取物都表明抗氧化活性通过显著的DPPH反映，TPC,FRAP,β-胡萝卜素分别为-69.13±3.00、1148.92±14.01、752.44±10.30和65.74±3.28。然而,对于TPC，分离的茶黄素表现出更高的抗氧化能力，分别为737.74±12.55、82.60±2.33和853.77±9.55，DPPH,和FRAP,分别,与提取物相比。在15天内，坐骨神经损伤和坐骨神经损伤物理诱导了疗效，并用离体茶黄素治疗。将总共12只健康的白化病小鼠随机分配到对照组（n=6）或茶黄素（5.0mg/kg（n=6））组。在这些群体中,行为测试用于评估和比较增强的功能恢复以及骨骼肌质量测量。血清样品包括氧化应激标志物。在茶黄素叶中，行为测试显示感觉运动功能恢复有统计学意义（p<.001）的改善，肌肉质量恢复，TOS大幅下降，TAC大幅增加，并增强抗氧化酶活性。考虑到茶黄素的上述治疗观点，目前的研究计划优化从红茶中分离茶黄素，并探讨其在小鼠模型中的神经保护作用。
