PDF(Pubmed)
Abstract：
Ninjinyoeito, Hochuekkito, and Juzentaihoto are the three types of Kampo-hozai used to support the treatment of various diseases by energizing patients through improved mental health. While Kampo-hozais are clinically used to improve mental energy decline, a comparison between their effects on neuropsychiatric symptoms like anxiety and sociability and the strength of their effects has not been conducted. Therefore, this study compared the effects of Ninjinyoeito, Hochuekkito, and Juzentaihoto on psychiatric symptoms using neuropeptide Y knockout (NPY-KO) zebrafish, a suitable animal model for anxiety and low sociability. Neuropeptide Y knockout zebrafish were fed a Ninjinyoeito, Hochuekkito, or Juzentaihoto-supplemented diet for 4 days. Then, sociability was analyzed using a three-Chambers test and anxiety-like behavior was evaluated using the cold stress and novel tank tests. The results showed that Ninjinyoeito treatment improved the low sociability of neuropeptide Y knockout, while Hochuekkito and Juzentaihoto did not. Neuropeptide Y knockout exhibited anxiety-like behaviors, such as freezing and swimming in the wall area under cold stress, but Ninjinyoeito treatment improved these behaviors. However, these anxiety-like behaviors were not improved by Hochuekkito and Juzentaihoto. Ninjinyoeito treatment also improved anxiety-like behaviors of neuropeptide Y knockout in the novel tank test. However, no improvement was shown in the Hochuekkito and Juzentaihoto groups. This trend was also confirmed in the low water stress test using wild-type zebrafish. This study exhibits that among the three types of Kampo-hozai, Ninjinyoeito is the most effective in psychiatric disorders associated with anxiety and low sociability.
摘要：
Ninjinyoeito,Hochuekkito,和Juzentaihoto是三种类型的Kampo-hozai，用于通过改善心理健康来激励患者，从而支持各种疾病的治疗。虽然Kampo-hozais在临床上用于改善精神能量下降，尚未对它们对神经精神症状如焦虑和社交能力的影响及其影响的强度进行比较。因此,这项研究比较了Ninjinyoeito的影响，Hochuekkito,和Juzentaihoto使用神经肽Y基因敲除（NPY-KO）斑马鱼的精神症状，一个适合焦虑和低社交能力的动物模型。神经肽Y敲除的斑马鱼被喂食Ninjinyoeito，Hochuekkito,或Juzentaihoto补充饮食4天。然后，使用三室测试分析社交能力，并使用冷压力和新颖的坦克测试评估焦虑样行为。结果表明，Ninjinyoeito治疗改善了神经肽Y基因敲除的低社交性，而Hochuekkito和Juzentaihoto没有。神经肽Y敲除表现出焦虑样行为，例如在寒冷的压力下在墙壁区域冻结和游泳，但是Ninjinyoeito治疗改善了这些行为。然而,Hochuekkito和Juzentaihoto并未改善这些焦虑样行为.Ninjinyoeito治疗还改善了新型坦克试验中神经肽Y敲除的焦虑样行为。然而,Hochuekkito和Juzentaihoto组无改善.在使用野生型斑马鱼的低水胁迫试验中也证实了这种趋势。这项研究表明，在三种类型的Kampo-hozai中，Ninjinyoeito是最有效的与焦虑和低社交能力相关的精神疾病。
