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Abstract：
Objective Lipemia is an important cause of preanalytical errors in laboratory results. They affect the specimen integrity and trustworthiness of laboratory results. The present study was to assess the impact of lipemia on routine clinical chemistry analytes. Methods Anonymous leftover serum samples with normal levels of routine biochemical parameters were pooled. Twenty such pooled serum samples were used for the study. The samples were spiked with commercially available intralipid solution (20%) to produce lipemic concentrations of 0, 400 (mild, 20 μL), 1,000 (moderate, 50 μL), and 2,000 mg/dL (severe, 100 μL). Glucose, renal function test, electrolytes, and liver function test were estimated in all the samples. Baseline data without the effect of interference was considered as true value and percentage bias for the spiked samples was calculated. Interference was considered significant if the interference bias percentage exceeded 10%. Result Parameters like glucose, urea, creatinine, direct bilirubin, sodium, potassium, and chloride showed negative interference at mild and moderate lipemic concentration and positive interference at severe lipemic concentration. Parameters like aspartate transaminase (AST) and alanine transaminase (ALT) showed negative interference at mild and positive interference at moderate and severe lipemic concentration. Whereas uric acid, total protein, albumin, total bilirubin, alkaline phosphatase, gamma-glutamyl transferase, calcium, magnesium, and phosphorous showed positive interference at all concentrations. Significant interference (> 10%) was shown for magnesium (mild lipemia), albumin, direct bilirubin, ALT, and AST at moderate lipemic concentration. All parameters showed significant interference at severe lipemic concentration. Conclusion All the study parameters are affected by lipemic interference at varying levels. Laboratory-specific data regarding lipemic interference at various concentrations on the clinical biochemistry parameters is needed.
摘要：
目的血脂症是实验室结果分析前错误的重要原因。它们影响样本的完整性和实验室结果的可信性。本研究旨在评估血脂对常规临床化学分析物的影响。方法收集常规生化指标正常的匿名剩余血清样本。将20个这样的汇集的血清样品用于研究。将样品掺入市售的脂质内溶液（20％），以产生0,400（轻度，20μL),1,000（中等，50μL),和2,000mg/dL（严重，100μL)。葡萄糖,肾功能试验,电解质，对所有样本进行肝功能检测。没有干扰影响的基线数据被认为是真实值,并且计算加标样品的百分比偏差。如果干扰偏差百分比超过10%,则认为干扰是显著的。结果参数像葡萄糖,尿素,肌酐,直接胆红素,钠,钾，氯在轻度和中度血脂浓度下显示负干扰，在重度血脂浓度下显示正干扰。天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）等参数在轻度和中度和重度血脂浓度下显示出负干扰。而尿酸，总蛋白质，白蛋白,总胆红素,碱性磷酸酶,γ-谷氨酰转移酶，钙，镁，磷在所有浓度下都表现出正干扰。对镁（轻度脂血）有显著干扰（>10%），白蛋白,直接胆红素,ALT,和AST在中等血脂浓度。在严重的血脂浓度下，所有参数均显示出明显的干扰。结论所有研究参数都受到不同程度的血脂干扰的影响。需要有关各种浓度对临床生化参数的血脂干扰的实验室特定数据。
