PDF(Pubmed)
Abstract：
Gastrointestinal symptoms are common health problems found during aging and neurodegenerative diseases. Trimethyltin-induced rat is known as an animal model of hippocampal degeneration with no data on enteric neurodegeneration. This study aimed to investigate the effect of trimethyltin (TMT) induction on the gastrointestinal tract. A 28-day animal study with male Sprague-Dawley rats (3 months old, 150-200 g) given a single TMT injection (8 mg/kg body weight, intraperitoneal) was conducted. The number of neurons in the colonic myenteric plexus was measured using stereological estimation. Histological scoring of colon inflammation, immunohistochemistry of tumor necrosis factor-α (TNF-α), and quantitative PCR were conducted. This study showed neuronal loss in the colonic myenteric plexus of TMT-induced rat model of neurodegeneration. Minor colon inflammation characterized by inflammatory cell infiltration and slightly higher expression of TNF-α in the colon mucosa were observed in the TMT-induced rat. However, the gut microbiota composition of the TMT-induced rat was not different from that of the control rats. This study demonstrates that TMT induces colonic myenteric plexus neurodegeneration and minor colon inflammation, which suggests the potential of this animal model to elucidate the communication between the gastrointestinal tract and central nervous system in neurodegenerative diseases.
摘要：
胃肠道症状是衰老和神经退行性疾病期间发现的常见健康问题。已知三甲基锡诱导的大鼠是海马变性的动物模型,没有关于肠神经变性的数据。本研究旨在探讨三甲基锡(TMT)诱导对胃肠道的影响。对雄性Sprague-Dawley大鼠（3个月大，150-200g）单次注射TMT（8mg/kg体重，腹膜内)进行。使用体视学估计测量结肠肌间神经丛中的神经元数量。结肠炎症的组织学评分，肿瘤坏死因子-α（TNF-α）的免疫组织化学，并进行定量PCR。这项研究表明TMT诱导的神经变性大鼠模型的结肠肌间神经丛神经元丢失。在TMT诱导的大鼠中观察到轻微的结肠炎症，其特征是炎症细胞浸润和结肠粘膜中TNF-α的表达略高。然而,TMT诱导的大鼠的肠道菌群组成与对照大鼠没有差异。这项研究表明，TMT诱导结肠肌间神经丛神经变性和轻微的结肠炎症，这表明这种动物模型有可能阐明神经退行性疾病中胃肠道与中枢神经系统之间的联系。
