PDF(Pubmed)
Abstract：
Pseudomonas aeruginosa causes urinary tract infections associated with catheters by forming biofilms on the surface of indwelling catheters. Therefore, controlling the spread of the bacteria is crucial to preventing its transmission in hospitals and the environment. Thus, our objective was to determine the antibiotic susceptibility profiles of twenty-five P. aeruginosa isolates from UTIs at the Medical Center of Trás-os-Montes and Alto Douro (CHTMAD). Biofilm formation and motility are also virulence factors studied in this work. Out of the twenty-five P. aeruginosa isolates, 16% exhibited multidrug resistance, being resistant to at least three classes of antibiotics. However, the isolates showed a high prevalence of susceptibility to amikacin and tobramycin. Resistance to carbapenem antibiotics, essential for treating infections when other antibiotics fail, was low in this study, Notably, 92% of the isolates demonstrated intermediate sensitivity to ciprofloxacin, raising concerns about its efficacy in controlling the disease. Genotypic analysis revealed the presence of various β-lactamase genes, with class B metallo-β-lactamases (MBLs) being the most common. The blaNDM, blaSPM, and blaVIM-VIM2 genes were detected in 16%, 60%, and 12% of the strains, respectively. The presence of these genes highlights the emerging threat of MBL-mediated resistance. Additionally, virulence gene analysis showed varying prevalence rates among the strains. The exoU gene, associated with cytotoxicity, was found in only one isolate, while other genes such as exoS, exoA, exoY, and exoT had a high prevalence. The toxA and lasB genes were present in all isolates, whereas the lasA gene was absent. The presence of various virulence genes suggests the potential of these strains to cause severe infections. This pathogen demonstrated proficiency in producing biofilms, as 92% of the isolates were found to be capable of doing so. Currently, antibiotic resistance is one of the most serious public health problems, as options become inadequate with the continued emergence and spread of multidrug-resistant strains, combined with the high rate of biofilm production and the ease of dissemination. In conclusion, this study provides insights into the antibiotic resistance and virulence profiles of P. aeruginosa strains isolated from human urine infections, highlighting the need for continued surveillance and appropriate therapeutic approaches.
摘要：
铜绿假单胞菌通过在留置导管的表面上形成生物膜而引起与导管相关的尿路感染。因此,控制细菌的传播对于防止其在医院和环境中的传播至关重要。因此，我们的目的是在Trás-os-Montes和AltoDouro医疗中心(CHTMAD)确定来自尿路感染的25种铜绿假单胞菌的抗生素敏感性.生物膜形成和运动性也是这项工作中研究的毒力因子。在25个铜绿假单胞菌分离物中,16%表现出多药耐药性，对至少三类抗生素有抗药性。然而,分离株对阿米卡星和妥布霉素的敏感性较高。耐碳青霉烯类抗生素,当其他抗生素失效时，对治疗感染至关重要，在这项研究中很低，值得注意的是,92%的分离株对环丙沙星有中等敏感性，引起人们对其控制疾病的功效的担忧。基因型分析显示存在各种β-内酰胺酶基因，B类金属-β-内酰胺酶（MBL）是最常见的。BlaNDM,blaSPM,BlaVIM-VIM2基因检测到16%，60%,12%的菌株，分别。这些基因的存在凸显了MBL介导的抗性的新威胁。此外,毒力基因分析显示菌株之间的流行率不同。exoU基因,与细胞毒性相关，只在一个分离物中发现,而其他基因如exoS，exoA,exoY,exoT的患病率很高。toxA和lasB基因存在于所有分离物中，而lasA基因缺失。各种毒力基因的存在表明这些菌株可能导致严重感染。这种病原体表现出生产生物膜的能力，因为92%的分离株被发现能够这样做。目前,抗生素耐药性是最严重的公共卫生问题之一，随着多重耐药菌株的持续出现和传播，选择变得不足，结合生物膜的高生产率和易于传播。总之,这项研究提供了对从人类尿液感染中分离出的铜绿假单胞菌的抗生素耐药性和毒力谱的见解，强调需要持续监测和适当的治疗方法。
