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Abstract：
Ovule number (ON) produced during flower development determines the maximum number of seeds per silique and thereby affects crop productivity; however, the genetic basis of ON remains poorly understood in oilseed rape (Brassica napus). In this study, we genetically dissected the ON variations in a double haploid (DH) population and in natural population (NP) by linkage mapping and genome-wide association analysis. Phenotypic analysis showed that ON displayed normal distribution in both populations with the broad-sense heritability of 0.861 (DH population) and 0.930 (natural population). Linkage mapping identified 5 QTLs related to ON, including qON-A03, qON-A07, qON-A07-2, qON-A10, and qON-C06. Genome-wide association studies (GWAS) revealed 214, 48, and 40 significant single-nucleotide polymorphisms (SNPs) by individually using the single-locus model GLM and the multiple-locus model MrMLM and FASTMrMLM. The phenotypic variation explained (PVE) by these QTLs and SNPs ranged from 2.00-17.40% to 5.03-7.33%, respectively. Integration of the results from both strategies identified four consensus genomic regions associated with ON from the chromosomes A03, A07, and A10. Our results preliminarily resolved the genetic basis of ON and provides useful molecular markers for plant yield improvement in B. napus.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s11032-023-01355-7.
UNASSIGNED: The online version contains supplementary material available at 10.1007/s11032-023-01355-7.
摘要：
花发育过程中产生的胚珠数（ON）决定了每个角果的最大种子数，从而影响作物生产力；但是，在油菜（甘蓝型油菜）中，ON的遗传基础仍然知之甚少。在这项研究中,我们通过连锁作图和全基因组关联分析,对双单倍体(DH)种群和自然种群(NP)中的ON变异进行了遗传学分析.表型分析表明，ON在两个种群中均表现出正态分布，广义遗传力分别为0.861（DH种群）和0.930（自然种群）。链接映射确定了与ON相关的5个QTL，包括qON-A03、qON-A07、qON-A07-2、qON-A10和qON-C06。通过单独使用单基因座模型GLM和多基因座模型MrMLM和FASTMrMLM，全基因组关联研究（GWAS）揭示了214、48和40个显着的单核苷酸多态性（SNP）。这些QTL和SNP解释的表型变异（PVE）范围为2.00-17.40％至5.03-7.33％，分别。来自两种策略的结果的整合鉴定了来自染色体A03、A07和A10的与ON相关的四个共有基因组区域。我们的结果初步解决了ON的遗传基础，并为油菜植物产量的提高提供了有用的分子标记。
■在线版本包含补充材料，可在10.1007/s11032-023-01355-7获得。
■在线版本包含补充材料，可在10.1007/s11032-023-01355-7获得。
