PDF(Pubmed)
Abstract：
Introduction. Triple negative breast carcinomas are characterized by a lack of hormone receptor and HER2 expression and inconsistent expression of breast-specific immunohistochemical markers. The expression of many site-specific markers in these tumors is largely unknown. The objective of the study was to examine the expression of widely used immunohistochemical markers on a large cohort of triple negative breast cancer. Methods. Sections from tissue microarrays were stained with 47 markers using routine protocols. Most markers were scored using a modified Allred method. ATRX, BAP1, SMAD4, e-cadherin, and beta-catenin were scored as retained or lost. Mammaglobin was considered positive if there was at least moderate intensity staining in any tumor cells. P16 was scored as overexpressed or not overexpressed; p53 was scored as wildtype, overexpressed, null, or cytoplasmic. Results. The cohort consisted of 639 tumors including 601 primary and 32 metastases. Overall, 96% expressed GATA3, mammaglobin, and/or SOX10 while 97% of no special type tumors expressed this panel. Carcinoma of apocrine differentiation demonstrated an AR positive, SOX10 negative, K5 negative/focal immunophenotype. PAX8 (SP348), WT1, Napsin A, and TTF1 (8G7G3/1) were never or rarely expressed while CA9, CDX2, NKX3.1, SATB2 (SATBA410), synaptophysin, and vimentin were variably expressed. Conclusions. Almost all TNBC express at least 1 of the 3 IHC markers: GATA3, mammaglobin, and/or SOX10. Carcinoma of apocrine differentiation is characterized by an AR positive, SOX10 negative, K5 negative or focal immunophenotype. Cautious interpretation of so-called site-specific markers, with knowledge of antibody clones, is required in excluding the diagnosis of triple negative breast cancer.
摘要：
Introduction.三阴性乳腺癌的特征是缺乏激素受体和HER2表达以及乳腺特异性免疫组织化学标志物的表达不一致。许多位点特异性标志物在这些肿瘤中的表达在很大程度上是未知的。该研究的目的是检查广泛使用的免疫组织化学标志物在大型三阴性乳腺癌队列中的表达。方法。使用常规方案将来自组织微阵列的切片用47个标记物染色。使用改良的Allred方法对大多数标记进行评分。ATRX,BAP1，SMAD4，e-cadherin，和β-catenin评分为保留或丢失。如果在任何肿瘤细胞中存在至少中等强度的染色,则认为乳腺球蛋白是阳性的。P16被评分为过表达或未过表达；p53被评分为野生型，过度表达，null,或者细胞质。结果。该队列由639个肿瘤组成，包括601个原发性和32个转移。总的来说,96%表达GATA3、乳腺珠蛋白、和/或SOX10，而97％的无特殊类型肿瘤表达该组。大汗腺分化癌显示AR阳性，SOX10阴性,K5阴性/局灶性免疫表型。PAX8(SP348),WT1,NapsinA,和TTF1（8G7G3/1）从未或很少表达，而CA9，CDX2，NKX3.1，SATB2（SATBA410），突触素,和波形蛋白表达不同。Conclusions.几乎所有TNBC都表达3种IHC标记中的至少1种：GATA3，乳腺球蛋白，和/或SOX10。大汗腺分化癌的特征是AR阳性，SOX10阴性,K5阴性或局灶性免疫表型。对所谓的位点特异性标记的谨慎解释，有抗体克隆的知识，排除三阴性乳腺癌的诊断是必需的。
