Abstract:
Basil (Ocimum basilicum L.) is among the most widely used aromatic plants of Lamiaceae, often grown in areas where salinity is an adverse factor. Most studies on the effect of salinity on basil focused on the influence of salt stress on productive traits, while few reported on how it affects the phytochemical composition and the aroma profile. Three basil cultivars (Dark Opal, Italiano Classico, and Purple Ruffles) were grown hydroponically for 34 days with two nutrient solutions that differed in NaCl concentration [no NaCl (Control) and 60 mM NaCl]. Yield, secondary metabolite concentration (β-carotene and lutein), antioxidant activity [1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferric reduction antioxidant power (FRAP)], and aroma profile based on composition of volatile organic compounds (VOCs) were appraised in response to salinity applications. Salt stress significantly reduced fresh yield in Italiano Classico and Dark Opal by 43.34 and 31.69%, respectively, while no effect was observed in Purple Ruffles. Furthermore, the salt-stress treatment increased β-carotene and lutein concentrations, DPPH, and FRAP activities, and the total nitrogen content of the latter cultivar. CG-MS analysis revealed significant differences in VOCs composition of the basil cultivars, with Italiano Classico and Dark Opal characterized by the predominance of linalool (average 37.52%), which, however, was negatively affected by salinity. In Purple Ruffles, the predominant VOC compound, estragole (79.50%), was not affected by the deleterious effects of NaCl-induced stress.
摘要:
罗勒(OcimumbasilicumL.)是唇形科最广泛使用的芳香植物之一,通常生长在盐度是不利因素的地区。大多数关于盐度对罗勒影响的研究集中在盐胁迫对生产性状的影响上,虽然很少报道它如何影响植物化学成分和香气。三个罗勒品种(暗蛋白石,ItalianoClassico,和紫色荷叶边)用两种NaCl浓度不同的营养液水培生长34天[无NaCl(对照)和60mMNaCl]。产量,次级代谢物浓度(β-胡萝卜素和叶黄素),抗氧化活性[1,1-二苯基-2-吡啶酰肼(DPPH)和三价铁还原抗氧化能力(FRAP)],根据盐度应用评估了基于挥发性有机化合物(VOCs)组成的香气特征。盐胁迫使ItalianoClassico和DarkOpal的新鲜产量显着降低了43.34%和31.69%,分别,而在紫色荷叶例中没有观察到效果。此外,盐胁迫处理增加了β-胡萝卜素和叶黄素的浓度,DPPH,和FRAP活动,和后一个品种的总氮含量。CG-MS分析显示,罗勒品种的VOCs组成存在显着差异,ItalianoClassico和DarkOpal的特征是芳樟醇占主导地位(平均37.52%),which,然而,受到盐度的负面影响。在紫色荷叶边,主要的VOC化合物,estragole(79.50%),不受NaCl诱导胁迫的有害影响。