PDF(Pubmed)
Abstract：
Nowadays, plant genetic resources are often at risk of loss and destruction. Geophytes are herbaceous or perennial species that are annually renewed by bulbs, rhizomes, tuberous roots, or tubers. They are often subject to overexploitation, which, combined with other biotic and abiotic stresses, can make these plants more vulnerable to a decline in their diffusion. As a result, multiple endeavors have been undertaken to establish better conservation strategies. Plant cryopreservation at ultra-low temperatures in liquid nitrogen (-196 °C) has proven to be an effective, long-term, low-cost, and suitable conservation method for many plant species. Over the last two decades, major advances in cryobiology studies have enabled successful explants of multiple genera and types, including pollen, shoot tips, dormant buds, and zygotic and somatic embryos. This review provides an update on recent advances and developments in cryopreservation and its application to medicinal and ornamental geophytes. In addition, the review includes a brief summary of factors limiting the success of bulbous germplasm conservation. The critical analysis underpinning this review will benefit biologists and cryobiologists in their further studies on the optimization of geophyte cryopreservation protocols and will support a more complete and wider application of knowledge in this area.
摘要：
如今,植物遗传资源往往面临损失和破坏的风险。地球植物是草本或多年生物种，每年由鳞茎更新，根茎,块茎根，或者块茎.它们经常受到过度开发，which,与其他生物和非生物胁迫相结合，会使这些植物更容易受到扩散下降的影响。因此,已经进行了多项努力来建立更好的保护策略。在液氮（-196°C）中超低温下进行植物冷冻保存已被证明是一种有效的，长期的,低成本,以及适用于许多植物物种的保护方法。在过去的二十年里，冷冻生物学研究的重大进展使多个属和类型的外植体得以成功，包括花粉,射击技巧，休眠的芽，以及合子和体细胞胚胎。本文综述了冷冻保存及其在药用和观赏性地植物中的应用的最新进展和发展。此外,这篇综述简要总结了限制球茎种质保存成功的因素。本综述的关键分析将使生物学家和冷冻生物学家进一步研究地球植物冷冻保存方案的优化，并将支持该领域更完整和更广泛的知识应用。
