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Abstract：
Red ginseng and Ophiopogon japonicus are both traditional Chinese medicines. They have also been used as food in China for thousands of years. These two herbs were frequently used in many traditional Chinese patent medicines. However, the carbohydrate compositions of these two herbs were not normally used during the production of said medicine, such as Shenmai injection, resulting in a large amount of waste composed of carbohydrates. In this study, the extraction conditions were optimized by response surface methodology. The Shenmai injection waste polysaccharide was extracted by using distilled water that was boiled under the optimized conditions. The Shenmai injection waste polysaccharide (SMP) was thereby obtained. SMP was further purified by anion exchange chromatography and gel filtration. With this method, a neutral polysaccharide fraction (SMP-NP) and an acidic polysaccharide fraction (SMP-AP) were obtained. The results of structure elucidation indicated that SMP-NP was a type of levan, and SMP-AP was a typical acidic polysaccharide. SMP-NP exhibited potential stimulation activity on the proliferation of five different Lactobacilli strains. Therefore, SMP-AP could promote the antioxidant defense of IPEC-J2 cells. These findings suggest that Shenmai injection waste could be used as a resource for prebiotics and antioxidants.
摘要：
红参和麦冬都是中药。它们在中国也被用作食物已有数千年的历史。这两种草药在许多传统中成药中经常使用。然而,这两种草药的碳水化合物成分在生产所述药物期间通常不使用，如参麦注射液,导致大量由碳水化合物组成的废物。在这项研究中,采用响应面法优化提取条件。采用在优化条件下煮沸的蒸馏水提取参麦注射液废多糖。由此得到参麦注射液废多糖(SMP)。通过阴离子交换色谱和凝胶过滤进一步纯化SMP。使用这种方法，获得中性多糖级分(SMP-NP)和酸性多糖级分(SMP-AP)。结构鉴定结果表明SMP-NP是一种左旋，SMP-AP是典型的酸性多糖。SMP-NP对5种不同的乳杆菌菌株的增殖表现出潜在的刺激活性。因此,SMP-AP可以促进IPEC-J2细胞的抗氧化防御。这些发现表明，参麦注射液废物可以用作益生元和抗氧化剂的资源。
