Abstract：
The endogenous regenerative capacity of the brain is quite weak; however, a regenerative reaction, the production of new neurons (neurogenesis), has been reported to occur in brain lesions. In addition, leukocytes are well known to infiltrate brain lesions. Therefore, leukocytes would also have a link with regenerative neurogenesis; however, their role has not been fully elucidated. In this study, we investigated leukocyte infiltration and its influence on brain tissue regeneration in a trimethyltin (TMT)-injected mouse model of hippocampal regeneration. Immunohistochemically, CD3-positive T lymphocytes were found in the hippocampal lesion of TMT-injected mice. Prednisolone (PSL) treatment inhibited T lymphocyte infiltration and increased neuronal nuclei (NeuN)-positive mature neurons and doublecortin (DCX)-positive immature neurons in the hippocampus. Investigation of bromodeoxyuridine (BrdU)-labeled newborn cells revealed the percentage of BrdU/NeuN- and BrdU/DCX-positive cells increased by PSL treatment. These results indicate that infiltrated T lymphocytes prevent brain tissue regeneration by inhibiting hippocampal neurogenesis.
摘要：
大脑的内源性再生能力相当弱；然而，再生反应，新神经元的产生（神经发生），据报道发生在脑部病变中。此外,众所周知，白细胞会渗入脑部病变。因此,白细胞也与再生神经发生有关；然而，其作用尚未完全阐明。在这项研究中,我们研究了三甲基锡（TMT）注射的海马再生小鼠模型中白细胞浸润及其对脑组织再生的影响。免疫组织化学，在注射TMT的小鼠的海马病变中发现了CD3阳性T淋巴细胞。泼尼松龙（PSL）治疗抑制了T淋巴细胞浸润，并增加了海马中神经元核（NeuN）阳性成熟神经元和doublecortin（DCX）阳性未成熟神经元。溴脱氧尿苷（BrdU）标记的新生细胞的研究显示，PSL处理增加了BrdU/NeuN-和BrdU/DCX阳性细胞的百分比。这些结果表明，浸润的T淋巴细胞通过抑制海马神经发生来阻止脑组织再生。
