PDF(Pubmed)
Abstract：
Autologous and allogeneic bone grafts remain the gold standard for repairing bone defects. However, donor shortages and postoperative infections contribute to unsatisfactory treatment outcomes. Tissue engineering technology that utilizes biologically active composites to accelerate the healing and reconstruction of segmental bone defects has led to new ideas for in situ bone repair. Multifunctional nanocomposite hydrogels were constructed by covalently binding silver (Ag+) core-embedded mesoporous silica nanoparticles (Ag@MSN) to bone morphogenetic protein-2 (BMP-2), which was encapsulated into silk fibroin methacryloyl (SilMA) and photo-crosslinked to form an Ag@MSN-BMP-2/SilMA hydrogel to preserve the biological activity of BMP-2 and slow its release. More importantly, multifunctional Ag+-containing nanocomposite hydrogels showed antibacterial properties. These hydrogels possessed synergistic osteogenic and antibacterial effects to promote bone defect repair. Ag@MSN-BMP-2/SilMA exhibited good biocompatibility in vitro and in vivo owing to its interconnected porosity and improved hydrophilicity. Furthermore, the multifunctional nanocomposite hydrogel showed controllable sustained-release activity that promoted bone regeneration in repairing rat skull defects by inducing osteogenic differentiation and neovascularization. Overall, Ag@MSN-BMP-2/SilMA hydrogels enrich bone regeneration strategies and show great potential for bone regeneration.
摘要：
自体和同种异体骨移植仍然是修复骨缺损的金标准。然而,供体短缺和术后感染导致治疗结果不理想.利用生物活性复合材料加速节段性骨缺损的愈合和重建的组织工程技术为原位骨修复带来了新思路。通过将银（Ag）核心嵌入的介孔二氧化硅纳米颗粒（Ag@MSN）共价结合到骨形态发生蛋白2（BMP-2）上，构建了多功能纳米复合水凝胶，将其包封到丝素蛋白甲基丙烯酰基（SilMA）中并光交联以形成Ag@MSN-BMP-2/SilMA水凝胶，以保持BMP-2的生物活性并减缓其释放。更重要的是,多功能含银纳米复合水凝胶具有抗菌性能。这些水凝胶具有协同成骨和抗菌作用以促进骨缺损修复。Ag@MSN-BMP-2/SilMA由于其互连的孔隙率和改善的亲水性而在体外和体内表现出良好的生物相容性。此外，多功能纳米复合水凝胶显示出可控的缓释活性，通过诱导成骨分化和新生血管形成促进大鼠颅骨缺损修复中的骨再生。总的来说,Ag@MSN-BMP-2/SilMA水凝胶丰富了骨再生策略，显示出骨再生的巨大潜力。
