PDF(Pubmed)
Abstract：
Mesenchymal stem/stromal cells (MSCs)-based therapy brings the reassuring capability to regenerative medicine through their self-renewal and multilineage potency. Also, they secret a diversity of mediators, which are complicated in moderation of deregulated immune responses, and yielding angiogenesis in vivo. Nonetheless, MSCs may lose biological performance after procurement and prolonged expansion in vitro. Also, following transplantation and migration to target tissue, they encounter a harsh milieu accompanied by death signals because of the lack of proper tensegrity structure between the cells and matrix. Accordingly, pre-conditioning of MSCs is strongly suggested to upgrade their performances in vivo, leading to more favored transplantation efficacy in regenerative medicine. Indeed, MSCs ex vivo pre-conditioning by hypoxia, inflammatory stimulus, or other factors/conditions may stimulate their survival, proliferation, migration, exosome secretion, and pro-angiogenic and anti-inflammatory characteristics in vivo. In this review, we deliver an overview of the pre-conditioning methods that are considered a strategy for improving the therapeutic efficacy of MSCs in organ failures, in particular, renal, heart, lung, and liver.
摘要：
基于间充质干细胞/基质细胞（MSC）的疗法通过其自我更新和多谱系潜能为再生医学带来了令人放心的能力。此外，他们隐藏着各种各样的调解人，在调节失调的免疫反应方面很复杂，并在体内产生血管生成。尽管如此，在体外获得和延长扩增后,MSC可能丧失生物学性能。此外，移植和迁移到靶组织后，由于细胞和基质之间缺乏适当的张力结构，他们遇到了伴随死亡信号的严酷环境。因此，强烈建议对MSCs进行预处理以提高其体内性能，导致再生医学中更受欢迎的移植功效。的确,MSCs体外缺氧预处理,炎症刺激，或其他因素/条件可能刺激他们的生存，扩散,迁移,外泌体分泌，和体内促血管生成和抗炎特性。在这次审查中,我们概述了预处理方法，这些方法被认为是提高MSCs在器官衰竭中的治疗效果的策略。特别是,肾,心,肺，还有肝脏.
