Abstract：
In recent years, sex-controlled breeding has emerged as an effective strategy to enhance the yields of economic animals with different growth characteristics, while increasing the economic benefits of aquaculture. It is known that the NF-κB pathway participates in gonadal differentiation and reproduction. Therefore, we used the large-scale loach as a research model for the present study and selected an effective inhibitor of the NF-κB signaling pathway (QNZ). This, to investigates the impacts of the NF-κB signaling pathway on gonadal differentiation during a critical period of gonad development and after maturation. Simultaneously, the sex ratio bias and the reproductive capacities of adult fish were analyzed. Our results indicated that the inhibition of the NF-κB signaling pathway influenced the expression of genes related to gonad development, regulated the gene expression related to the brain-gonad-liver axis of juvenile loaches, and finally impacted the gonadal differentiation of the large-scale loach and promoted a male-biased sex ratio. Meanwhile, high QNZ concentrations affected the reproductive abilities of adult loaches and inhibited the growth performance of offspring. Thus, our results deepened the exploration of sex control in fish and provided a certain research basis for the sustainable development of the aquaculture industry.
摘要：
近年来,性别控制育种已成为提高具有不同生长特征的经济动物产量的有效策略，同时增加了水产养殖的经济效益。已知NF-κB途径参与性腺分化和生殖。因此,本研究以大规模的泥法子为研究模型,筛选出一种有效的NF-κB信号通路抑制剂(QNZ)。这个,探讨NF-κB信号通路在性腺发育关键时期和成熟后对性腺分化的影响。同时,分析了成年鱼的性别比例偏差和繁殖能力。我们的结果表明NF-κB信号通路的抑制影响了性腺发育相关基因的表达,调节与幼年泥沙脑-性腺-肝轴相关的基因表达，最终影响了大规模泥ach的性腺分化，并促进了男性偏向的性别比例。同时,高QNZ浓度会影响成年泥桃的生殖能力，并抑制后代的生长性能。因此，结果深化了对鱼类性别控制的探索,为水产养殖业的可持续发展提供了一定的研究依据。
