Abstract：
Despite significant advances in diabetes management, particularly with the introduction of the most recent continuous glucose monitoring devices (CGMDs) that can monitor glucose actively in the transdermal interstitial fluid (ISF) in vivo, CGMDs still have significant disadvantages in terms of accuracy, low interference effect, precision, and stability. This is mostly because they detect hydrogen peroxide at higher potentials and require an oxygen-rich environment. First in its class, we developed an oxygen-insensitive polymeric glucose microneedle (MN) that was functionalized using a new electron-transfer mediator, 3-(3\'-phenylimino)-3H-phenothiazinesulfonic acid-based enzyme cocktail for the NAD-GDH system. The inclusion of reduced graphene oxide aided in the absorption of the cocktail via the π-π interaction and enhanced the conductivity and sensor performance. The MN exhibited a dynamic linear range (1-30 mM) with a low detection limit of 26 μM, high sensitivity (18.05 μAmM-1 cm-2), stability (up to 7 days), high selectivity (due to a low oxidation potential of 0.15 V), and a fast response time (∼3 s). In vivo, deployment of the MN in a rabbit model demonstrated that the ISF glucose concentrations measured with the MN for up to 24 h correlate very well with the blood glucose concentrations measured with a commercial glucometer.
摘要：
尽管糖尿病管理取得了重大进展，特别是引入了最新的连续葡萄糖监测设备（CGMD），可以在体内主动监测经皮间质液（ISF）中的葡萄糖，CGMD在准确性方面仍然有明显的缺点，低干扰效果，精度,和稳定性。这主要是因为它们在较高电势下检测过氧化氢并且需要富氧环境。在班上第一个，我们开发了一种对氧不敏感的聚合物葡萄糖微针（MN），它使用一种新的电子转移介质进行了功能化，用于NAD-GDH系统的基于3-(3'-苯基亚氨基)-3H-苯噻嗪磺酸的酶混合物。包含还原的氧化石墨烯有助于通过π-π相互作用吸收混合物，并增强了电导率和传感器性能。MN表现出动态线性范围（1-30mM），低检测限为26μM，高灵敏度（18.05μAMM-1cm-2），稳定性（最长7天），高选择性（由于0.15V的低氧化电位），和快速的响应时间(〜3s)。在体内,在兔模型中部署MN表明，用MN测量长达24小时的ISF葡萄糖浓度与用商业血糖仪测量的血糖浓度非常好地相关。
