PDF(Pubmed)
Abstract：
UNASSIGNED: Ex vivo confocal microscopy is a real-time technique that provides high-resolution images of fresh, non-fixed tissues, with an optical resolution comparable to conventional pathology. The objective of this study was to investigate the feasibility of using ex vivo confocal microscopy in fusion mode (FuCM) and the haematoxylin and eosin (H&E)-like digital staining that results for the analysis of basic patterns of lesion in nephropathology.
UNASSIGNED: Forty-eight renal samples were scanned in a fourth-generation ex vivo confocal microscopy device. Samples were subjected to confocal microscopy imaging and were then processed using conventional pathology techniques. Concordance between the techniques was evaluated by means of the percentage of agreement and the κ index.
UNASSIGNED: Agreement between conventional microscopy and H&E-like digital staining was strong (κ = 0.88) in the evaluation of acute tubular damage and was substantial (κ = 0.79) in the evaluation of interstitial fibrosis, interstitial inflammation, arterial and arteriolar lesions. H&E-like digital staining also allows rapid identification of extracapillary proliferation (κ = 0.88), necrosis and segmental sclerosis (κ = .88) in the glomerular compartment, but the results reported here are limited because of the small number of cases with these glomerular findings.
UNASSIGNED: FuCM proved to be as effective as conventional techniques in evaluating the presence of acute tubular necrosis and interstitial fibrosis changes, but in fresh tissue. The ease of acquisition of ex vivo confocal microscopy images suggests that FuCM may be useful for rapid evaluation of kidney biopsies and to restructure the clinical workflow in renal histopathology.
UNASSIGNED: Forty-eight renal samples were scanned in a fourth-generation ex vivo confocal microscopy device. Samples were subjected to confocal microscopy imaging and were then processed using conventional pathology techniques. Concordance between the techniques was evaluated by means of the percentage of agreement and the κ index.
UNASSIGNED: Agreement between conventional microscopy and H&E-like digital staining was strong (κ = 0.88) in the evaluation of acute tubular damage and was substantial (κ = 0.79) in the evaluation of interstitial fibrosis, interstitial inflammation, arterial and arteriolar lesions. H&E-like digital staining also allows rapid identification of extracapillary proliferation (κ = 0.88), necrosis and segmental sclerosis (κ = .88) in the glomerular compartment, but the results reported here are limited because of the small number of cases with these glomerular findings.
UNASSIGNED: FuCM proved to be as effective as conventional techniques in evaluating the presence of acute tubular necrosis and interstitial fibrosis changes, but in fresh tissue. The ease of acquisition of ex vivo confocal microscopy images suggests that FuCM may be useful for rapid evaluation of kidney biopsies and to restructure the clinical workflow in renal histopathology.
摘要：
■离体共聚焦显微镜是一种实时技术，可提供新鲜，非固定组织,具有与常规病理学相当的光学分辨率。这项研究的目的是研究在融合模式（FuCM）和苏木精和伊红（H＆E）样数字染色中使用离体共聚焦显微镜的可行性，这些数字染色用于分析肾病病变的基本模式。
■在第四代离体共聚焦显微镜装置中扫描了48个肾脏样本。对样品进行共聚焦显微镜成像,然后使用常规病理学技术进行处理。通过一致性百分比和κ指数评估技术之间的一致性。
■常规显微镜和H&E样数字染色在急性肾小管损伤的评估中具有很强的一致性（κ=0.88），在间质纤维化的评估中具有实质性的一致性（κ=0.79）。间质性炎症,动脉和小动脉病变。H&E-likedigital染色法还可以快速鉴定毛细血管外增殖（κ=0.88），肾小球小室坏死和节段性硬化（κ=.88），但是由于这些肾小球发现的病例数量很少，因此此处报告的结果有限。
■FuCM在评估急性肾小管坏死和间质纤维化变化方面与常规技术一样有效，而是在新鲜的组织里.易于获取离体共聚焦显微镜图像表明FuCM可用于快速评估肾脏活检和重组肾脏组织病理学的临床工作流程。
■在第四代离体共聚焦显微镜装置中扫描了48个肾脏样本。对样品进行共聚焦显微镜成像,然后使用常规病理学技术进行处理。通过一致性百分比和κ指数评估技术之间的一致性。
■常规显微镜和H&E样数字染色在急性肾小管损伤的评估中具有很强的一致性（κ=0.88），在间质纤维化的评估中具有实质性的一致性（κ=0.79）。间质性炎症,动脉和小动脉病变。H&E-likedigital染色法还可以快速鉴定毛细血管外增殖（κ=0.88），肾小球小室坏死和节段性硬化（κ=.88），但是由于这些肾小球发现的病例数量很少，因此此处报告的结果有限。
■FuCM在评估急性肾小管坏死和间质纤维化变化方面与常规技术一样有效，而是在新鲜的组织里.易于获取离体共聚焦显微镜图像表明FuCM可用于快速评估肾脏活检和重组肾脏组织病理学的临床工作流程。
