PDF(Pubmed)
Abstract：
Beta carotene is a natural anti-oxidant agent, and it inhibits the matrix metalloprotease (MMP) activity. Diabetic neuropathic pain (DNP) is produced by cellular oxidative stress. The role of the beta carotene effect in diabetic neuropathic pain is not explored yet. The present study is designed for the evaluation of the palm oil mill effluent-derived beta carotene (PBC) effect in DNP in zebrafish. The DNP was induced by the intraperitoneal administration of streptozotocin (STZ). Blood glucose levels of above 15 mM were considered to be diabetic conditions. The zebrafish were exposed to test compound PBC (25, 50, and 100 µM), pregabalin (PG: 10 μM), and an MMP-13 inhibitor (CL-82198; 10 μM) for 10 consecutive days from day 11. The neuralgic behavioral parameters, i.e., temperature test, acetic acid test, and fin clip test were assessed on day 0 and the 7th, 14th, and 21st days. On the 22nd day, the blood glucose and MMP-13 levels and brain thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH), and MMP-13 activity levels were estimated. The treatment of PBC ameliorated the DNP-associated behavioral and biochemical changes. The results are similar to those of PG and CL-82198 treatments. Hence, the PBC possesses a potentially ameliorative effect against DNP due to its potential anti-oxidant, anti-lipid peroxidation, and MMP-13 inhibitory actions.
摘要：
β-胡萝卜素是一种天然抗氧化剂,抑制基质金属蛋白酶(MMP)活性。糖尿病性神经性疼痛(DNP)由细胞氧化应激产生。β-胡萝卜素效应在糖尿病性神经性疼痛中的作用尚未探索。本研究旨在评估棕榈油厂废水中β-胡萝卜素（PBC）对斑马鱼DNP的影响。通过腹膜内施用链脲佐菌素(STZ)诱导DNP。高于15mM的血糖水平被认为是糖尿病病症。斑马鱼暴露于测试化合物PBC（25、50和100µM），普瑞巴林（PG：10μM），和MMP-13抑制剂(CL-82198;10μM),从第11天开始连续10天。神经痛行为参数，即，温度测试,乙酸试验,在第0天和第7天评估鳍夹测试，14日,第21天。第22天,血糖和MMP-13水平和脑硫代巴比妥酸反应物质（TBARS），还原型谷胱甘肽（GSH），并估计MMP-13活性水平。PBC的治疗改善了DNP相关的行为和生化改变。结果与PG和CL-82198处理的结果相似。因此，由于其潜在的抗氧化剂，PBC对DNP具有潜在的改善作用，抗脂质过氧化，和MMP-13抑制作用。
