Abstract：
OBJECTIVE: To investigate the potential mechanisms underlying the migration of endogenous neural stem cells (eNSCs) to the frontal cortex to differentiate into neurons, and to monitor the effect of electroacupuncture (EA) regulation of focal cerebral ischemia (FCI) in rats on the expression of growth arrest-specific protein 7 (Gas7) and nerve growth factor (NGF) in the prefrontal cortex (PFC).
METHODS: Randomly, forty-eight male Sprague-Dawley rats were divided into four groups: Normal, Sham operation, Model, and EA. The right middle cerebral artery was embolized utilizing the thread-embolism technique. In the EA group, \"Baihui\" and \"Zusanli\" points were treated with electroacupuncture for 30 minutes, once a day, for 21 days. Nissl staining revealed the neuronal morphology of the PFC. Using immunohistochemistry and Western blot, the expression of Gas7 and NGF in the right PFC was observed.
RESULTS: Nissl staining showed clear PFC neurons with centered nuclei and distinct nucleoli in the Normal and Sham groups. In the Model group, the PFC nuclei were distinctively smaller. The neuronal morphology in the EA group resembled that of the Normal group. Results from Western blot and immunohistochemistry were comparable. The expression of Gas7 and NGF in the Sham surgery group did not differ significantly from the Normal group. However, the expression of Gas7 and NGF in the Model group was significantly lower than in the Normal group. The expression of Gas7 and NGF was significantly higher in the EA group than in the Model group.
CONCLUSIONS: EA can increase the expressions of Gas7 and NGF in the ischemic prefrontal cortex, which may be one of the mechanisms by which EA promotes the differentiation of eNSCs into neurons in the injured area.
METHODS: Randomly, forty-eight male Sprague-Dawley rats were divided into four groups: Normal, Sham operation, Model, and EA. The right middle cerebral artery was embolized utilizing the thread-embolism technique. In the EA group, \"Baihui\" and \"Zusanli\" points were treated with electroacupuncture for 30 minutes, once a day, for 21 days. Nissl staining revealed the neuronal morphology of the PFC. Using immunohistochemistry and Western blot, the expression of Gas7 and NGF in the right PFC was observed.
RESULTS: Nissl staining showed clear PFC neurons with centered nuclei and distinct nucleoli in the Normal and Sham groups. In the Model group, the PFC nuclei were distinctively smaller. The neuronal morphology in the EA group resembled that of the Normal group. Results from Western blot and immunohistochemistry were comparable. The expression of Gas7 and NGF in the Sham surgery group did not differ significantly from the Normal group. However, the expression of Gas7 and NGF in the Model group was significantly lower than in the Normal group. The expression of Gas7 and NGF was significantly higher in the EA group than in the Model group.
CONCLUSIONS: EA can increase the expressions of Gas7 and NGF in the ischemic prefrontal cortex, which may be one of the mechanisms by which EA promotes the differentiation of eNSCs into neurons in the injured area.
摘要：
目的：探讨内源性神经干细胞（eNSCs）向额叶皮质分化为神经元的潜在机制，并监测电针(EA)调节大鼠局灶性脑缺血(FCI)对前额叶皮质(PFC)生长阻滞特异性蛋白7(Gas7)和神经生长因子(NGF)表达的影响。
方法：随机，将48只雄性Sprague-Dawley大鼠分为四组：正常，假操作,型号,和EA。使用线栓术栓塞右大脑中动脉。在EA组中,“百会”和“足三里”穴位用电针治疗30分钟，一天一次,21天Nissl染色显示PFC的神经元形态。使用免疫组织化学和蛋白质印迹，在右侧PFC中观察到Gas7和NGF的表达。
结果：Nissl染色显示在正常组和Sham组中具有中心核和明显核仁的清晰PFC神经元。在“模型”组中，PFC核明显较小。EA组中的神经元形态类似于正常组。Westernblot和免疫组织化学的结果具有可比性。假手术组Gas7和NGF的表达与正常组没有明显差别。然而,模型组Gas7和NGF的表达明显低于正常组。EA组Gas7和NGF的表达明显高于模子组。
结论：EA可增加缺血前额叶皮质Gas7和NGF的表达，这可能是EA促进神经干细胞分化为受损区域神经元的机制之一。
方法：随机，将48只雄性Sprague-Dawley大鼠分为四组：正常，假操作,型号,和EA。使用线栓术栓塞右大脑中动脉。在EA组中,“百会”和“足三里”穴位用电针治疗30分钟，一天一次,21天Nissl染色显示PFC的神经元形态。使用免疫组织化学和蛋白质印迹，在右侧PFC中观察到Gas7和NGF的表达。
结果：Nissl染色显示在正常组和Sham组中具有中心核和明显核仁的清晰PFC神经元。在“模型”组中，PFC核明显较小。EA组中的神经元形态类似于正常组。Westernblot和免疫组织化学的结果具有可比性。假手术组Gas7和NGF的表达与正常组没有明显差别。然而,模型组Gas7和NGF的表达明显低于正常组。EA组Gas7和NGF的表达明显高于模子组。
结论：EA可增加缺血前额叶皮质Gas7和NGF的表达，这可能是EA促进神经干细胞分化为受损区域神经元的机制之一。
