PDF(Pubmed)
Abstract：
Female pigs respond sensitive both to DON and ZEN with anorexia and endocrine disruption, respectively, when critical diet concentrations are exceeded. Therefore, the frequent co-contamination of feed by DON and ZEN requires their parallel inactivation. The additive ZenA hydrolyzes ZEN while SBS inactivates DON through sulfonation. Both supplements were simultaneously added (+, 2.5 g SBS and 100 U ZenA/kg) to a control diet (CON-, 0.04 mg DON and < 0.004 mg ZEN/kg; CON+, 0.03 mg DON and < 0.004 mg ZEN/kg) and a Fusarium toxin contaminated diet (FUS-, 2.57 mg DON and 0.24 mg ZEN/kg; FUS+, 2.04 mg DON and 0.24 mg ZEN/kg). The 4 diets were fed to 20 female weaned piglets each (6 kg initial body weight) for 35 days; the piglets were sacrificed thereafter for collecting samples. Supplements improved performance and modified metabolism and hematology independent of dietary DON contamination. The mechanisms behind these changes could not be clarified and require further consideration. SBS reduced DON concentration in feed by approximately 20% and to the same extent in blood plasma and urine suggesting that no further DON sulfonate formation occurred in the digestive tract before absorbing DON in the upper digestive tract or that additionally formed DON sulfonates escaped absorption. DON sulfonates were detected in feces suggesting that unabsorbed DON sulfonates reached feces and/or that unabsorbed DON was sulfonated in the hindgut. The observed reduction rate of 20% was evaluated to be insufficient for feeding practice. Galenic form of SBS added to dry feed needs to be improved to support the DON sulfonation in the proximal digestive tract.ZenA was active in the digestive tract as demonstrated by the presence of its hydrolyzed none-estrogenic reaction products hydrolyzed ZEN (HZEN) and decarboxylated and hydrolyzed ZEN (DHZEN) both in feces, systemic circulation, and urine of group FUS+ compared to group FUS-. The presence of these hydrolysis products was paralleled by a significant decrease in high-estrogenic ZEN concentrations which, in turn, was related to a decrease in relative weights of uteri and ovaries when compared to group FUS-. Thus, ZenA was proven to be effective; both in terms of biomarkers and biological effects.
摘要：
雌性猪对DON和ZEN反应敏感，伴有厌食和内分泌干扰，分别,当超过临界饮食浓度时。因此,DON和ZEN对饲料的频繁共污染需要它们的平行灭活。添加剂ZenA水解ZEN,而SBS通过磺化使DON失活。同时添加两种补充剂（+，2.5克SBS和100UZenA/kg)与对照饮食(CON-，0.04mgDON和<0.004mgZEN/kg；CON+，0.03mgDON和<0.004mgZEN/kg）和镰刀菌毒素污染的饮食（FUS-，2.57mgDON和0.24mgZEN/kg；FUS+，2.04mgDON和0.24mgZEN/kg）。将4种日粮饲喂20只雌性断奶仔猪(初始体重6kg)35天;此后处死仔猪以收集样品。补充剂改善了性能，改善了代谢和血液学，而与饮食DON污染无关。这些变化背后的机制无法澄清，需要进一步考虑。SBS将饲料中的DON浓度降低了约20％，并在血浆和尿液中降低了相同程度，这表明在上消化道吸收DON之前，消化道中没有进一步形成DON磺酸盐，或者另外形成的DON磺酸盐逃脱了吸收。在粪便中检测到DON磺酸盐，表明未吸收的DON磺酸盐到达粪便和/或未吸收的DON在后肠中被磺化。观察到的20%的减少率被评估为不足以用于喂养实践。添加到干饲料中的SBS的Galenic形式需要改进以支持近端消化道中的DON磺化。ZenA在消化道中具有活性，其水解的非雌激素反应产物水解的ZEN（HZEN）和脱羧和水解的ZEN（DHZEN）在粪便中均存在，全身循环，FUS+组尿液与FUS-组相比。这些水解产物的存在伴随着高雌激素ZEN浓度的显着降低，反过来,与FUS组相比，与子宫和卵巢的相对重量降低有关。因此，ZenA被证明是有效的;在生物标志物和生物效应方面。
