PDF(Pubmed)
Abstract：
Neurofilament light chain (NfL) is an axonal cytoplasmic protein in neurons. Recently, NfL has shown potential as a diagnostic biomarker in dogs with meningoencephalitis of unknown origin (MUO). However, there have been no studies on the biomarkers of lesion progression and resolution in MUO.
To identify the potential of NfL as a biomarker for predicting changes in lesions.
Seven dogs with MUO who had undergone two magnetic resonance imaging (MRI) scans were included. The serum NfL levels were measured using a single-molecule array. The relationship between the rate of change in lesion size and the rate of change in serum NfL level was analysed using simple linear regression. To investigate the effect of changes in lesion size on NfL levels, the dogs were divided into two groups depending on the change in lesion size: decreased lesion size group (n = 5) and increased lesion size group (n = 2). Trends in lesion size change were identified in the second MRI compared with the first MRI.
A significant positive relationship between the rate of lesion size change and the rate of NfL level change was identified (R2 = 0.9239, p = 0.0006). In the decreased lesion size group (n = 5), all NfL levels in each dog decreased, and in the increased lesion size group (n = 2), all NfL levels in each dog increased.
This preliminary study showed a positive relationship between the rate of change in lesion size and rate of change in serum NfL levels. Therefore, the serum NfL level may be a promising biomarker of lesion progression and resolution in MUO.
To identify the potential of NfL as a biomarker for predicting changes in lesions.
Seven dogs with MUO who had undergone two magnetic resonance imaging (MRI) scans were included. The serum NfL levels were measured using a single-molecule array. The relationship between the rate of change in lesion size and the rate of change in serum NfL level was analysed using simple linear regression. To investigate the effect of changes in lesion size on NfL levels, the dogs were divided into two groups depending on the change in lesion size: decreased lesion size group (n = 5) and increased lesion size group (n = 2). Trends in lesion size change were identified in the second MRI compared with the first MRI.
A significant positive relationship between the rate of lesion size change and the rate of NfL level change was identified (R2 = 0.9239, p = 0.0006). In the decreased lesion size group (n = 5), all NfL levels in each dog decreased, and in the increased lesion size group (n = 2), all NfL levels in each dog increased.
This preliminary study showed a positive relationship between the rate of change in lesion size and rate of change in serum NfL levels. Therefore, the serum NfL level may be a promising biomarker of lesion progression and resolution in MUO.
摘要：
背景：神经丝轻链（NfL）是神经元中的轴突细胞质蛋白。最近,NfL已显示出作为患有未知来源的脑膜脑炎（MUO）的狗的诊断生物标志物的潜力。然而,目前尚无关于MUO中病变进展和消退的生物标志物的研究.
目的：确定NfL作为预测病变变化的生物标志物的潜力。
方法：纳入7只MUO犬，他们接受了两次磁共振成像（MRI）扫描。使用单分子阵列测量血清NfL水平。采用简单线性回归分析病灶大小变化率与血清NfL水平变化率的关系。为了研究病变大小的变化对NfL水平的影响，根据病变大小的变化，将狗分为两组：病变大小减小组（n=5）和病变大小增加组（n=2）。与第一次MRI相比，第二次MRI中发现了病变大小变化的趋势。
结果：确定了病变大小变化率与NfL水平变化率之间的显着正相关关系（R2=0.9239，p=0.0006）。在病变大小减小组（n=5）中，每只狗的所有NfL水平都下降了，在病变大小增加组（n=2）中，每只狗的所有NfL水平都增加了。
结论：这项初步研究表明，病变大小的变化率与血清NfL水平的变化率之间存在正相关关系。因此,血清NfL水平可能是MUO病变进展和消退的有希望的生物标志物。
目的：确定NfL作为预测病变变化的生物标志物的潜力。
方法：纳入7只MUO犬，他们接受了两次磁共振成像（MRI）扫描。使用单分子阵列测量血清NfL水平。采用简单线性回归分析病灶大小变化率与血清NfL水平变化率的关系。为了研究病变大小的变化对NfL水平的影响，根据病变大小的变化，将狗分为两组：病变大小减小组（n=5）和病变大小增加组（n=2）。与第一次MRI相比，第二次MRI中发现了病变大小变化的趋势。
结果：确定了病变大小变化率与NfL水平变化率之间的显着正相关关系（R2=0.9239，p=0.0006）。在病变大小减小组（n=5）中，每只狗的所有NfL水平都下降了，在病变大小增加组（n=2）中，每只狗的所有NfL水平都增加了。
结论：这项初步研究表明，病变大小的变化率与血清NfL水平的变化率之间存在正相关关系。因此,血清NfL水平可能是MUO病变进展和消退的有希望的生物标志物。
