PDF(Pubmed)
Abstract：
The magnitude of the childhood obesity epidemic and its effects on public health has accelerated the pursuit of practical preventative measures. Epigenetics is one subject that holds a lot of promise, despite being relatively new. The study of potentially heritable variations in gene expression that do not require modifications to the underlying DNA sequence is known as epigenetics. Here, we used Illumina MethylationEPIC BeadChip Array to identify differentially methylated regions in DNA isolated from saliva between normal weight (NW) and overweight/obese (OW/OB) children and between European American (EA) and African American (AA) children. A total of 3133 target IDs (associated with 2313 genes) were differentially methylated (p < 0.05) between NW and OW/OB children. In OW/OB children, 792 target IDs were hypermethylated and 2341 were hypomethylated compared to NW. Similarly, in the racial groups EA and AA, a total of 1239 target IDs corresponding to 739 genes were significantly differentially methylated in which 643 target IDs were hypermethylated and 596 were hypomethylated in the AA compared to EA participants. Along with this, the study identified novel genes that could contribute to the epigenetic regulation of childhood obesity.
摘要：
儿童肥胖流行的规模及其对公共卫生的影响加速了对实际预防措施的追求。表观遗传学是一个有很多希望的学科，尽管相对较新。对不需要对基础DNA序列进行修饰的基因表达中的潜在可遗传变异的研究被称为表观遗传学。这里,我们使用Illumina甲基化EPICBeadChipArray在正常体重(NW)和超重/肥胖(OW/OB)儿童之间以及在欧美(EA)和非洲裔(AA)儿童之间从唾液中分离出的DNA中鉴定差异甲基化区域.在NW和OW/OB儿童之间，共有3133个靶ID（与2313个基因相关）差异甲基化（p<0.05）。在OW/OB子项中，与NW相比，792个目标ID被高甲基化，2341个目标ID被低甲基化。同样，在EA和AA种族群体中，与EA参与者相比,在AA中,共有1239个对应于739个基因的靶ID显著差异甲基化,其中643个靶ID高甲基化,596个低甲基化.伴随着这个,这项研究发现了可能有助于儿童肥胖表观遗传调控的新基因。
