PDF(Pubmed)
Abstract：
Farming seabass (Dicentrarchus labrax) is an essential activity in the Mediterranean basin including the Aegean Sea. The main seabass producer is Turkey accounting for 155,151 tons of production in 2021. In this study, skin swabs of seabass farmed in the Aegean Sea were analysed with regard to the isolation and identification of Pseudomonas. Bacterial microbiota of skin samples (n = 96) from 12 fish farms were investigated using next-generation sequencing (NGS) and metabarcoding analysis. The results demonstrated that Proteobacteria was the dominant bacterial phylum in all samples. At the species level, Pseudomonas lundensis was identified in all samples. Pseudomonas, Shewanella, and Flavobacterium were identified using conventional methods and a total of 46 viable (48% of all NGS+) Pseudomonas were isolated in seabass swab samples. Additionally, antibiotic susceptibility was determined according to standards of the European Committee on Antimicrobial Susceptibility Testing (EUCAST) and Clinical and Laboratory Standards Institute (CLSI) in psychrotrophic Pseudomonas. Pseudomonas strains were tested for susceptibility to 11 antibiotics (piperacillin-tazobactam, gentamicin, tobramycin, amikacin, doripenem, meropenem, imipenem, levofloxacin, ciprofloxacin, norfloxacin, and tetracycline) from five different groups of antibiotics (penicillins, aminoglycosides, carbapenems, fluoroquinolones, and tetracyclines). The antibiotics chosen were not specifically linked to usage by the aquaculture industry. According to the EUCAST and CLSI, three and two Pseudomonas strains were found to be resistant to doripenem and imipenem (E-test), respectively. All strains were susceptible to piperacillin-tazobactam, amikacin, levofloxacin, and tetracycline. Our data provide insight into different bacteria that are prevalent in the skin microbiota of seabass sampled from the Aegean Sea in Turkey, and into the antibiotic resistance of psychrotrophic Pseudomonas spp.
摘要：
在包括爱琴海在内的地中海盆地，耕种海底（Dicentrarchuslabrax）是一项必不可少的活动。主要的鲈鱼生产国是土耳其，2021年的产量为155,151吨。在这项研究中,分析了在爱琴海养殖的鲈鱼的皮肤拭子，以分离和鉴定假单胞菌。使用下一代测序（NGS）和代谢编码分析研究了来自12个养鱼场的皮肤样品（n=96）的细菌微生物群。结果表明，变形杆菌是所有样品中的优势细菌门。在物种层面,在所有样品中都鉴定了假单胞菌。假单胞菌,Shewanella,使用常规方法鉴定了黄杆菌属，并在鲈鱼拭子样品中分离出46种活的假单胞菌（占所有NGS的48％）。此外,抗生素敏感性是根据欧洲抗菌药物敏感性试验委员会(EUCAST)和临床和实验室标准研究所(CLSI)的标准在嗜冷假单胞菌中确定的.假单胞菌菌株对11种抗生素（哌拉西林-他唑巴坦，庆大霉素,妥布霉素,阿米卡星，多尼培南,美罗培南,亚胺培南,左氧氟沙星,环丙沙星,诺氟沙星,和四环素）来自五个不同的抗生素组（青霉素，氨基糖苷类,碳青霉烯类,氟喹诺酮类药物,和四环素）。选择的抗生素与水产养殖业的使用没有特别的联系。根据EUCAST和CLSI，发现三株和两株假单胞菌对多尼培南和亚胺培南耐药（E-test），分别。所有菌株对哌拉西林他唑巴坦敏感,阿米卡星，左氧氟沙星,还有四环素.我们的数据提供了对从土耳其爱琴海采样的鲈鱼皮肤微生物群中普遍存在的不同细菌的见解，以及嗜冷假单胞菌属的抗生素耐药性。
