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Abstract：
This study conducted transcriptome sequencing of goat-mammary-gland tissue at the late lactation (LL), dry period (DP), and late gestation (LG) stages to reveal the expression characteristics and molecular functions of circRNAs during mammary involution. A total of 11,756 circRNAs were identified in this study, of which 2528 circRNAs were expressed in all three stages. The number of exonic circRNAs was the largest, and the least identified circRNAs were antisense circRNAs. circRNA source gene analysis found that 9282 circRNAs were derived from 3889 genes, and 127 circRNAs\' source genes were unknown. Gene Ontology (GO) terms, such as histone modification, regulation of GTPase activity, and establishment or maintenance of cell polarity, were significantly enriched (FDR < 0.05), which indicates the functional diversity of circRNAs\' source genes. A total of 218 differentially expressed circRNAs were identified during the non-lactation period. The number of specifically expressed circRNAs was the highest in the DP and the lowest in LL stages. These indicated temporal specificity of circRNA expression in mammary gland tissues at different developmental stages. In addition, this study also constructed circRNA-miRNA-mRNA competitive endogenous RNA (ceRNA) regulatory networks related to mammary development, immunity, substance metabolism, and apoptosis. These findings help understand the regulatory role of circRNAs in mammary cell involution and remodeling.
摘要：
本研究对哺乳期晚期（LL）的山羊乳腺组织进行了转录组测序，干燥期（DP），和妊娠晚期（LG）阶段，以揭示乳腺退化过程中circRNAs的表达特征和分子功能。在这项研究中总共鉴定了11,756个circRNAs，其中2528个circRNAs在所有三个阶段都表达。外显子circRNAs的数量是最大的，鉴定最少的circRNAs是反义circRNAs。circRNA来源基因分析发现，9282个circRNAs来自3889个基因，和127个circRNAs\'源基因未知。基因本体论(GO)术语，如组蛋白修饰，GTP酶活性的调节，以及细胞极性的建立或维持，显著富集(FDR<0.05)，这表明circRNAs源基因的功能多样性。在非泌乳期鉴定了总共218个差异表达的circRNAs。特异性表达的circRNAs的数量在DP阶段最高，在LL阶段最低。这些表明circRNA在不同发育阶段的乳腺组织中表达的时间特异性。此外,本研究还构建了与乳腺发育相关的circRNA-miRNA-mRNA竞争性内源性RNA(ceRNA)调控网络,豁免权,物质代谢，和凋亡。这些发现有助于理解circRNAs在乳腺细胞退化和重塑中的调节作用。
