PDF(Pubmed)
Abstract：
We investigated the effects of CEC on the fermentation characteristics, epithelial gene expression, and bacterial community in the rumen of lambs fed a high-concentrate diet. Twenty-four 3-month-old female crossbred lambs with an initial body weight of 30.37 ± 0.57 kg were randomly allocated to consume a diet supplemented with 80 mg/kg CEC (CEC) or not (CON). The experiment consisted of a 14 d adaptation period and a 60 d data collection period. Compared with the CON group, the CEC group had higher ADG, epithelial cell thickness, ruminal butyrate proportion, and lower ammonia nitrogen concentration. Increases in the mRNA expression of Occludin and Claudin-4, as well as decreases in the mRNA expression of apoptotic protease activating factor-1 (Apaf-1), cytochrome c (Cyt-C), Caspase-8, Caspase-9, Caspase-3, Caspase-7, and toll-like receptor 4 (TLR4), were observed in the CEC group. Moreover, CEC treatment also decreased the concentration of IL-1β, IL-12, and TNF-α. Supplementation with CEC altered the structure and composition of the rumen bacterial community, which was indicated by the increased relative abundances of Firmicutes, Synergistota, Rikenellaceae_RC9_gut_group, Olsenella, Schwartzia, Erysipelotrichaceae_UCG-002, Lachnospiraceae_NK3A20_group, Acetitomaculum, [Eubacterium]_ruminantium_group, Prevotellaceae_UCG-004, Christensenellaceae_R-7_group, Sphaerochaeta, Pyramidobacter, and [Eubacterium]_eligens_group, and the decreased relative abundances of Acidobacteriota, Chloroflexi, Gemmatimonadota, and MND1. Furthermore, Spearman correlation analysis revealed that the altered rumen bacteria were closely correlated with rumen health-related indices. Dietary CEC supplementation improved growth performance, reduced inflammation and apoptosis, protected barrier function, and modulated the bacterial community of lambs fed a high-concentrate diet.
摘要：
我们研究了CEC对发酵特性的影响，上皮基因表达，和饲喂高浓度饮食的羔羊瘤胃中的细菌群落。随机分配24只3个月大的雌性杂交羔羊，其初始体重为30.37±0.57kg，以食用补充有80mg/kgCEC（CEC）或不补充CEC（CON）的饮食。实验包括14d的适应期和60d的数据收集期。与CON组相比,CEC组ADG较高,上皮细胞厚度，瘤胃丁酸盐比例，和较低的氨氮浓度。Occludin和Claudin-4的mRNA表达增加，以及凋亡蛋白酶活化因子-1（Apaf-1）的mRNA表达减少，细胞色素c(Cyt-C),Caspase-8、Caspase-9、Caspase-3、Caspase-7和toll样受体4(TLR4),在CEC组中观察到。此外，CEC处理也降低了IL-1β的浓度，IL-12和TNF-α。补充CEC改变了瘤胃细菌群落的结构和组成，Firmicutes的相对丰度增加表明了这一点，Synergistota,栗科_RC9_gut_组，Olsenella,Schwartzia,丹毒科_UCG-002,仙人掌科_NK3A20_组,活动带,[Eubacterium]_rumantium_group,Prevotellaceae_UCG-004，Christensenellaceae_R-7_组，Sphaerochaeta,吡喃杆菌,和[Eubacterium]_eligens_group，酸细菌的相对丰度下降，氯氟菌,Gemmatimonadota,MND1。此外，Spearman相关分析表明，瘤胃细菌改变与瘤胃健康相关指标密切相关。膳食补充CEC改善了生长性能，减少炎症和细胞凋亡，受保护的屏障功能，并调节饲喂高浓度饮食的羔羊的细菌群落。
