PDF(Pubmed)
Abstract：
An 8-year-old male Rhodesian Ridgeback was presented with fever and severe thrombocytopenia. Clinical and laboratory examination, echocardiography, blood culture, and pathohistology revealed evidence of infective endocarditis, ischemic renal infarcts, and septic encephalitis. Treatment was started immediately but the dog\'s condition worsened, and the dog had to be euthanized. The causative Streptococcus canis strain was detected by blood culture and MALDI-TOF MS and analyzed using whole-genome sequencing and multilocus sequence typing. Antibiotic susceptibility testing did not detect any resistance. The affected heart valve was analyzed using FISH imaging, which showed a streptococcal biofilm on the heart valve. Bacteria in biofilms are recalcitrant to antibiotic treatment. Early diagnosis could be beneficial to treatment outcome. Treatment of endocarditis could be improved by researching the optimal dosage of antibiotics in conjunction with the use of biofilm-active drugs.
摘要：
一名8岁的男性罗得西亚脊背出现发烧和严重的血小板减少症。临床和实验室检查，超声心动图,血培养,病理组织学显示了感染性心内膜炎的证据，缺血性肾梗死,和脓毒性脑炎.立即开始治疗，但狗的病情恶化，狗必须被安乐死.通过血液培养和MALDI-TOFMS检测致病犬链球菌菌株，并使用全基因组测序和多位点序列分型进行分析。抗生素敏感性测试未检测到任何耐药性。使用FISH成像分析受影响的心脏瓣膜，显示心脏瓣膜上有链球菌生物膜。生物膜中的细菌难以抵抗抗生素治疗。早期诊断可能有利于治疗结果。可以通过研究抗生素的最佳剂量以及使用生物膜活性药物来改善心内膜炎的治疗。
